Autocrine Regulation of the Renal Arterial Tone by Adrenomedullin
| Autor: | Hideo Kanaide, Joichi Kumazawa, Junji Nishimura, Sei Kobayashi, Hiroshi Seguchi |
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| Rok vydání: | 1995 |
| Předmět: |
Peptide Biosynthesis
medicine.medical_specialty Swine Molecular Sequence Data Biophysics Gene Expression In Vitro Techniques Biology Polymerase Chain Reaction Biochemistry Muscle Smooth Vascular Adrenomedullin Phenylephrine Renal Artery Internal medicine medicine Animals Homeostasis RNA Messenger Autocrine signalling Molecular Biology Antihypertensive Agents DNA Primers Fluorescent Dyes Messenger RNA Base Sequence Cell Biology Molecular biology Reverse transcriptase Reverse transcription polymerase chain reaction Endocrinology Cytosolic ca Muscle Tonus Potassium Calcium Fura-2 Peptides Muscle Contraction Hormone medicine.drug |
| Zdroj: | Biochemical and Biophysical Research Communications. 215:619-625 |
| ISSN: | 0006-291X |
| Popis: | We tested the hypothesis that adrenomedullin (ADM), a recently isolated hypotensive peptide, is an autocrine transmitter in renal arterial smooth muscle cells (RASMCs). Fura-2 fluorometry revealed that ADM decreases both the cytosolic Ca 2+ concentration ([Ca 2+ ]i) and tension induced by 0.3 μM phenylephrine (PE) in porcine RASMCs. The expression of ADM mRNA in RASMCs was assessed by the reverse transcription polymerase chain reaction (RT-PCR), using the total RNA from the RASMCs and the specific primers for the porcine ADM mRNA. A band with an expected size could be detected only when the reverse transcriptase was added, thus indicating that the porcine RASMCs express ADM mRNA. These results therefore suggested that ADM plays an important role in the regulation of the renal vascular tone, not only as a circulating hormone, but also as an autocrine transmitter. |
| Databáze: | OpenAIRE |
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