Transfusion of autologous late-outgrowth endothelial cells reduces arterial neointima formation after injury
| Autor: | Ai-Qun Kang, Yong-Xiao Cao, Xiao Ge Zhao, Xin Ma, Zeng-Li Li, Le Li, Shu-Qin Liu, Chuan-Hao Liu, Bing-Xiang Yuan |
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| Rok vydání: | 2010 |
| Předmět: |
Male
Neointima Pathology medicine.medical_specialty Time Factors Vascular smooth muscle Endothelium Physiology Angiogenesis Transplantation Autologous Muscle Smooth Vascular Cell Movement Physiology (medical) Paracrine Communication Cell Adhesion Animals Medicine Angiogenic Proteins Cells Cultured Cell Proliferation Hyperplasia business.industry Endothelial Cells Ear Arteries Vascular System Injuries Tunica intima Surgery Endothelial stem cell Disease Models Animal medicine.anatomical_structure Culture Media Conditioned Artery Endothelium Rabbits Bone marrow Inflammation Mediators Tunica Intima Cardiology and Cardiovascular Medicine business |
| Zdroj: | Cardiovascular Research. 90:171-181 |
| ISSN: | 1755-3245 0008-6363 |
| DOI: | 10.1093/cvr/cvq395 |
| Popis: | Aims Late-outgrowth endothelial cells (OECs) exist in blood and other organs. We aimed to explore whether and how OECs participate in re-endothelialization and prevent vascular neointima formation after injury. Methods and results Rabbit bone marrow OECs were cultured for 4 weeks to increase their numbers. Transfusion of autologous OECs (2 × 106–1 × 107/kg) soon after rabbit ear central artery injury reduced the increase in intima area and the decrease in lumen area observed at days 14 and 28. Transfusion of autologous OECs (1 × 107/kg) ameliorated some early (days 2 and 7) inflammatory and angiogenic responses (local and systemic) to the injury. Red fluorescence was seen within 7 days after transfusion of 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine-labelled acetylated low-density lipoprotein (Dil-acLDL)-incorporated OECs, and 1 h after perfusion of the isolated rabbit ear with Ringer–Locke solution containing Dil-acLDL-incorporated OECs, in the injured rabbit ear central artery. After transfusion of 5-bromo-2′-deoxyuridine (BrdU) incorporated autologous OECs, BrdU-positive cells appeared in the injured artery intima at day 3 and were present in the rescued artery endothelium at day 28. The OECs, ranging from 5%–15% of vascular smooth muscle cells (VSMCs), and the OEC-conditioned medium (5–15%) both inhibited VSMC proliferation and migration in vitro and regulated the arrangement of VSMCs. The VSMCs were helpful for OECs to form tubes in vitro . Conclusion Circulating OECs participate in re-endothelialization directly and inhibit VSMC migration and proliferation by a paracrine pathway; transfusion of large numbers of autologous OECs soon after vascular injury may prevent neointima formation. |
| Databáze: | OpenAIRE |
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