Extracellular matrix scaffold for cardiac repair
| Autor: | Jianhua Cui, Stephen F. Badylak, Megumi Mathison, Alka Redkar, Nicolas Chronos, Jin-Shen Li, Robert G. Matheny, Keith A. Robinson |
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| Rok vydání: | 2005 |
| Předmět: |
Male
Pathology medicine.medical_specialty Heart Ventricles Sus scrofa Urinary Bladder Myocardial Infarction Infarction Biocompatible Materials Matrix (biology) Extracellular matrix Tissue engineering Physiology (medical) Absorbable Implants Materials Testing medicine Animals Polytetrafluoroethylene Heart Failure Wound Healing Tissue Engineering business.industry Myocardium Prostheses and Implants medicine.disease Flow Cytometry Extracellular Matrix Heart failure Myocardial infarction complications Female Cardiology and Cardiovascular Medicine business Wound healing Biomarkers Calcification |
| Zdroj: | Circulation. 112 |
| ISSN: | 1524-4539 |
| Popis: | Background— Heart failure remains a significant problem. Tissue-engineered cardiac patches offer potential to treat severe heart failure. We studied an extracellular matrix scaffold for repairing the infarcted left ventricle. Methods and Results— Pigs (n=42) underwent left ventricular (LV) infarction. At 6 to 8 weeks, either 4-layer multilaminate urinary bladder-derived extracellular matrix or expanded polytetrafluoroethlyene (ePTFE) was implanted as full-thickness LV wall patch replacement. At 1-week, 1-month, or 3-month intervals, pigs were terminated. After macroscopic examination, samples of tissue were prepared for histology, immunocytochemistry, and analysis of cell proportions by flow cytometry. One-week and 1-month patches were intact with thrombus and inflammation; at 1 month, there was also tissue with spindle-shaped cells in proteoglycan-rich and collagenous matrix. More α-smooth muscle actin-positive cells were present in urinary bladder matrix (UBM) than in ePTFE (22.2±3.3% versus 8.4±2.7%; P =0.04). At 3 months, UBM was bioresorbed, and a collagen-rich vascularized tissue with numerous myofibroblasts was present. Isolated regions of α-sarcomeric actin-positive, intensely α-smooth muscle actin-immunopositive, and striated cells were observed. ePTFE at 3 months had foreign-body response with necrosis and calcification. Flow cytometry showed similarities of cells from UBM to normal myocardium, whereas ePTFE had limited cardiomyocyte markers. Conclusions— Appearance of a fibrocellular tissue that included contractile cells accompanied biodegradation of UBM when implanted as an LV-free wall infarction patch. UBM appears superior to synthetic material for cardiac patching and trends toward myocardial replacement at 3 months. |
| Databáze: | OpenAIRE |
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