| Autor: |
Jiaqiang Deng, Danting Li, Xiaoya Huang, Weiyao Li, Fangfang Zhao, Congwei Gu, Liuhong Shen, Suizhong Cao, Zhihua Ren, Zhicai Zuo, Junliang Deng, Shumin Yu |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
Molecular biology reports. 49(9) |
| ISSN: |
1573-4978 |
| Popis: |
The immunomodulatory function of mesenchymal stem cells (MSCs) has been considered to be vital for MSC-based therapies. Many works have been devoted to excavate effective strategies for enhancing the immunomodulation effect of MSCs. Nonetheless, canine MSC-mediated immunomodulation is still poorly understood.The inflammatory microenvironment was simulated through the employment of interferon-γ (IFN-γ) in a culture system. Compared with unstimulated cBMSCs, IFN-γ stimulation increased the mRNA levels of Toll-like receptor 3 (TLR3) and indoleamine 2, 3-dioxygenase 1 (IDO-1), and simultaneously enhanced the secretion of immunosuppressive molecules, including interleukin (IL)-10, hepatocyte growth factor (HGF), and kynurenine in cBMSCs. IFN-γ stimulation significantly enhanced the ability of cBMSCs and their supernatant to suppress the proliferation of murine spleen lymphocytes. Lymphocyte subtyping evaluation revealed that cBMSCs and their supernatant diminished the percentage of CD3Our findings substantiate that IFN-γ stimulation can enhance the immunomodulatory properties of cBMSCs by promoting TLR3-dependent activation of the IDO/kynurenine pathway, increasing the secretion of immunoregulatory molecules and strengthening interactions with T lymphocytes, which may provide a meaningful strategy for the clinical application of cBMSCs in immune-related diseases. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink