Roles of Multiple Flagellins in Flagellar Formation and Flagellar Growth Post Bdelloplast Lysis in Bdellovibrio bacteriovorus
| Autor: | Yoshiko Iida, R. Elizabeth Sockett, Shin-Ichi Aizawa, Andy Fenton, Carey Lambert, Laura Hobley |
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| Rok vydání: | 2009 |
| Předmět: |
Molecular Sequence Data
Mutant tapered wave Flagellum flagellin Article Bdellovibrio Protein filament Gene Knockout Techniques Bacterial Proteins Microscopy Electron Transmission Structural Biology Gene expression Electrophoresis Gel Two-Dimensional Amino Acid Sequence Molecular Biology Polyacrylamide gel electrophoresis EM electron microscopy RT reverse transcription biology Gene Expression Profiling Bdellovibrio bacteriovorus biology.organism_classification Molecular biology Cell biology Flagella biology.protein Sequence Alignment 2D gel polymorphism flagellin secretion Gene Deletion Flagellin |
| Zdroj: | Journal of Molecular Biology |
| ISSN: | 0022-2836 |
| Popis: | Bdellovibrio bacteriovorus cells have a single polar flagellum whose helical pitch and diameter characteristically change near the midpoint, resulting in a tapered wave. There are six flagellin genes in the genome: fliC1 to fliC6. Accordingly, the flagellar filament is composed of several similar flagellin species. We have used knockout mutants of each gene and analyzed the mutational effects on the filament length and on the composition and localization of each flagellin species in the filament by electron microscopy and one- and two-dimensional polyacrylamide gel electrophoresis. The location and amounts of flagellins in a filament were determined to be as follows: a small amount of FliC3 at the proximal end, followed by a large amount of FliC5, a large amount of FliC1, a small amount of FliC2 in this order, and a large amount of FliC6 at the distal end. FliC4 was present at a low level, but the location was not determined. Filament lengths of newly born progeny cells increased during prolonged incubation in nutrient-deficient buffer. The newly formed part of the elongated filament was composed of mainly FliC6. Reverse transcription PCR analysis of flagellar gene expression over 5 days in buffer showed that fliC gene expression tailed off over 5 days in the wild-type cells, but in the fliC5 mutant, expression of the fliC2, fliC4, and fliC6 genes was elevated on day 5, suggesting that they may be expressed to compensate for the absence of a major component, FliC5. |
| Databáze: | OpenAIRE |
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