Increased Plasma P-Selectin Induced by Intravenous Administration of Endotoxin in Rats
| Autor: | Emi Misugi, Shinichiro J. Tojo, Yukiko Kurata, Taeko Yasuda, Shigeaki Morooka |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Lipopolysaccharides
Male medicine.medical_specialty P-selectin Lipopolysaccharide Biophysics Enzyme-Linked Immunosorbent Assay Binding Competitive Biochemistry Rats Sprague-Dawley chemistry.chemical_compound Internal medicine medicine Animals Molecular Biology chemistry.chemical_classification medicine.diagnostic_test Chemistry Maximum level Cell Biology Reference Standards Molecular biology Rats Blot Disease Models Animal Kinetics P-Selectin Enzyme Endocrinology Solubility Immunoassay Injections Intravenous Injury model |
| Zdroj: | Biochemical and Biophysical Research Communications. 246:414-417 |
| ISSN: | 0006-291X |
| DOI: | 10.1006/bbrc.1998.8634 |
| Popis: | We developed a competitive enzyme immunoassay for detection of rat P-selectin and examined temporal changes of plasma P-selectin levels in an endotoxin-induced injury model in rats. Soluble P-selectin was detected in rat plasma after intravenous administration of lipopolysaccharide (LPS), and increased to a maximum level of five-fold over baseline after 24 hours. Plasma P-selectin was partially purified by gel-filtration and was identified as a 122 kDa band by Western blotting. Using the ELISA system we developed, monitoring of plasma P-selectin has become possible in the rat. |
| Databáze: | OpenAIRE |
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