Relationship between Proinflammatory and Antioxidant Proteins with the Severity of Cardiovascular Disease in Type 2 Diabetes Mellitus

Autor: Victoria Longobardo, Pedro Rozas-Moreno, Sonia Morales-Santana, Beatriz García-Fontana, Rebeca Reyes-García, Manuel Muñoz-Torres, José A. García-Salcedo
Přispěvatelé: Instituto de Salud Carlos III, Junta de Andalucía, European Commission, Consejo Superior de Investigaciones Científicas (España), Red Temática de Investigación Cooperativa en Envejecimiento y Fragilidad (España), [García-Fontana,B, Morales-Santana,S, Reyes-García,R, Muñoz-Torres,M] Bone Metabolic Unit, Endocrinology Division (RETICEF), Instituto de Investigación Biosanitaria (Ibs) Granada, University Hospital San Cecilio, Granada, Spain. [Morales-Santana,S] Proteomic Research Service, Instituto de Investigación Biosanitaria (Ibs) Granada, University Hospital San Cecilio, Granada, Spain. [Longobardo,V] Proteomic Research Service, Institute of Parasitology and Biomedicine 'López Neyra' (C.S.I.C.), Granada, Spain. [Rozas-Moreno,P] Endocrinology Division, Ciudad Real General Hospital, Ciudad Real, Spain. [García-Salcedo,JA] Infectious Diseases Unit, Instituto de Investigación Biosanitaria (Ibs) Granada, University Hospital San Cecilio, Granada, Spain. [Muñoz-Torres,M] Endocrinology Unit, University Hospital San Cecilio, Spain., This work was support in part by Consejería de Salud y Bienestar Social (Junta de Andalucía) Grant (PI05142012), RETICEF (RD06/0013/1014-RD12/0043/0014) and Fondo de Investigación Sanitaria (Instituto Carlos III) Grants (PI12/02141) with co-financing from FEDER (Fondo Europeo de Desarrollo Regional).
Rok vydání: 2015
Předmět:
Male
Proteomics
Analytical
Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Physical Examination::Anthropometry [Medical Subject Headings]
Antioxidantes
Named Groups::Persons::Age Groups::Adult::Middle Aged [Medical Subject Headings]
Disease
Analytical
Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Chemistry Techniques
Analytical::Mass Spectrometry::Spectrometry
Mass
Matrix-Assisted Laser Desorption-Ionization [Medical Subject Headings]
medicine.disease_cause
Severity of Illness Index
Antioxidants
Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans [Medical Subject Headings]
lcsh:Chemistry
Pathogenesis
Chemicals and Drugs::Chemical Actions and Uses::Pharmacologic Actions::Molecular Mechanisms of Pharmacological Action::Antioxidants [Medical Subject Headings]
Analytical
Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Chemistry Techniques
Analytical::Electrophoresis::Electrophoresis
Gel
Two-Dimensional::Two-Dimensional Difference Gel Electrophoresis [Medical Subject Headings]
Tandem Mass Spectrometry
Electrophoresis
Gel
Two-Dimensional
Phenomena and Processes::Metabolic Phenomena::Metabolism::Oxidative Stress [Medical Subject Headings]
Proinflammation
lcsh:QH301-705.5
Espectrometría de masas en tándem
Spectroscopy
education.field_of_study
Anthropometry
Chemistry
Cromatografía liquida
Estrés oxidativo
General Medicine
Middle Aged
Prognosis
Cardiovascular disease
Humanos
Computer Science Applications
Analytical
Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Epidemiologic Study Characteristics as Topic::Epidemiologic Studies::Cross-Sectional Studies [Medical Subject Headings]
Diabetes mellitus tipo 2
Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Oxidoreductases::Peroxidases::Glutathione Peroxidase [Medical Subject Headings]
Cardiovascular Diseases
Enfermedades cardiovasculares
Oxidation-Reduction
Analytical
Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Chemistry Techniques
Analytical::Mass Spectrometry::Tandem Mass Spectrometry [Medical Subject Headings]
Adult
medicine.medical_specialty
Disciplines and Occupations::Natural Science Disciplines::Biological Science Disciplines::Biochemistry::Proteomics [Medical Subject Headings]
Chemicals and Drugs::Biological Factors::Biomarkers [Medical Subject Headings]
Population
Espectrometría de masa por láser de matriz asistida de ionización desorción
Check Tags::Male [Medical Subject Headings]
Phenomena and Processes::Chemical Phenomena::Chemical Processes::Physicochemical Processes::Oxidation-Reduction [Medical Subject Headings]
Article
Catalysis
Proinflammatory cytokine
Diabetes Complications
Inorganic Chemistry
Internal medicine
Diabetes mellitus
Type 2 diabetes mellitus
Named Groups::Persons::Age Groups::Adult [Medical Subject Headings]
medicine
Humans
Physical and Theoretical Chemistry
education
Diseases::Cardiovascular Diseases [Medical Subject Headings]
Molecular Biology
Analytical
Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Chemistry Techniques
Analytical::Chromatography::Chromatography
Liquid [Medical Subject Headings]
Inflammation
Glutathione Peroxidase
Organic Chemistry
Diseases::Endocrine System Diseases::Diabetes Mellitus::Diabetes Mellitus
Type 2 [Medical Subject Headings]
Proteomic
Type 2 Diabetes Mellitus
Retinol-Binding Proteins
Cellular
Electroforesis bidimensional diferencial en gel
medicine.disease
Analytical
Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Data Collection::Health Surveys::Health Status Indicators::Severity of Illness Index [Medical Subject Headings]
Retinol binding protein
Biomarcadores
Proteómica
Cross-Sectional Studies
Endocrinology
lcsh:Biology (General)
lcsh:QD1-999
Diabetes Mellitus
Type 2
Oxidative stress
Spectrometry
Mass
Matrix-Assisted Laser Desorption-Ionization
Diseases::Endocrine System Diseases::Diabetes Mellitus::Diabetes Complications [Medical Subject Headings]
Analytical
Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Prognosis [Medical Subject Headings]
Diseases::Pathological Conditions
Signs and Symptoms::Pathologic Processes::Inflammation [Medical Subject Headings]
Biomarkers
Chromatography
Liquid
Zdroj: International Journal of Molecular Sciences
International Journal of Molecular Sciences, Vol 16, Iss 5, Pp 9469-9483 (2015)
Digital.CSIC. Repositorio Institucional del CSIC
instname
Volume 16
Issue 5
Pages 9469-9483
ISSN: 1422-0067
DOI: 10.3390/ijms16059469
Popis: Type 2 diabetes mellitus patients are at significant risk of cardiovascular disease, however, the pathophysiology of these complications is complex and incompletely known in this population. The aim of this study was to compare the serum proteome of patients with type 2 diabetes mellitus presenting or not presenting cardiovascular disease with non-diabetic subjects to find essential proteins related to these cardiovascular complications. This cross-sectional study compares the serum proteome by a combination of protein depletion with 2D-DIGE (2-dimension Difference Gel Electrophoresis) methodology. The proteins differentially expressed were identified by MALDI TOF/TOF (Matrix-assisted laser desorption/ionization and Time-Of-Flight ion detector) or LC-MS/MS (Liquid Chromatography coupled to Mass-Mass Spectrometry). Type 2 diabetes mellitus patients with cardiovascular disease showed higher expression of plasma retinol binding protein and glutathione peroxidase-3 compared to those without cardiovascular disease and non-diabetic controls. These results show that proteins related to the inflammatory and redox state appear to play an important role in the pathogenesis of the cardiovascular disease in the type 2 diabetes mellitus patients. © 2015 by the authors; licensee MDPI, Basel, Switzerland.
This work was support in part by Consejería de Salud y Bienestar Social (Junta de Andalucía) Grant (PI05142012), RETICEF (RD06/0013/1014-RD12/0043/0014) and Fondo de Investigación Sanitaria (Instituto Carlos III) Grants (PI12/02141) with co-financing from FEDER (Fondo Europeo de Desarrollo Regional). We acknowledge support by the CSIC Open Access Publication Initiative through its Unit of Information Resources for Research (URICI).
Databáze: OpenAIRE
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