Effects of dexamethasone on fetal hemoglobin synthesis in peripheral blood erythroid burst-forming units
| Autor: | M. B. Coleman, Annette Pressley, Hernan E. Grenett, Martin H. Steinberg, Fred A. Garver, Junius G. Adams |
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| Rok vydání: | 1981 |
| Předmět: |
congenital
hereditary and neonatal diseases and abnormalities medicine.medical_specialty Thalassemia Radioimmunoassay Anemia Sickle Cell Biology Dexamethasone Bone Marrow hemic and lymphatic diseases Internal medicine Fetal hemoglobin medicine Humans Globin Protein Precursors Cells Cultured Fetal Hemoglobin hemic and immune systems Hematology medicine.disease Hematopoietic Stem Cells Sickle cell anemia Globins Endocrinology Erythropoietin embryonic structures Erythropoiesis circulatory and respiratory physiology medicine.drug |
| Zdroj: | American journal of hematology. 10(1) |
| ISSN: | 0361-8609 |
| Popis: | Colonies derived from erythroid burst-forming units (BFU-E) synthesize fetal hemoglobin (HbF) in amounts that far exceed in vivo levels. There is some evidence that HbF synthesis is controlled at the level of a primitive erythroid precursor cell. Dexamethasone may potentiate the development of BFU-E. Since a means of augmenting HbF production in sickle cell anemia or severe beta-thalassemia would be of great therapeutic value, we studied the effects of dexamethasone on HbF and gamma-globin chain synthesis in BFU-E from patients with sickle cell anemia and controls. HbF was measured by radioimmunoassay of BFU-E lysate and gamma-chain synthesis by the incorporation of 3H-leucine into globin, which was then purified by gel filtration and column chromatography. Dexamethasone (10(-9) M) produced an increase in the number of BFU-E in 16 of 19 subjects when compared with numbers of BFU-E cultured with only erythropoietin. The individual BFU-E were larger and contained more subcolonies. Dexamethasone did not increase HbF or gamma-chain synthesis, and there was no relationship between proliferation of BFU-E and augmented HbF production. Thus, although dexamethasone augmented the development of erythroid bursts, there was no increment in HbF. |
| Databáze: | OpenAIRE |
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