Combination of two complementary automated rapid assays for diagnosis of heparin‐induced thrombocytopenia (HIT)
| Autor: | Na Li, Ishac Nazy, James W. Smith, Theodore E. Warkentin, Jane C. Moore, Donald M. Arnold, Jo-Ann I. Sheppard |
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| Rok vydání: | 2020 |
| Předmět: |
medicine.medical_specialty
Observational Trial medicine.drug_class Chemiluminescence immunoassay blood platelets thrombocytopenia 030204 cardiovascular system & hematology Platelet Factor 4 Monoclonal antibody Gastroenterology Immunoenzyme Techniques 03 medical and health sciences 0302 clinical medicine Internal medicine Heparin-induced thrombocytopenia medicine Humans immunoassay Platelet activation High probability medicine.diagnostic_test Heparin business.industry clinical laboratory techniques Anticoagulants Original Articles Hematology Platelet Activation medicine.disease PLATELETS Derivation cohort Immunoassay Original Article business |
| Zdroj: | Journal of Thrombosis and Haemostasis |
| ISSN: | 1538-7836 |
| DOI: | 10.1111/jth.14794 |
| Popis: | Background HIT diagnosis typically uses complementary diagnostic assays (eg, a PF4‐dependent enzyme‐immunoassay [EIA] and a platelet activation assay such as the serotonin‐release assay [SRA]). Objectives To determine whether the combination of two automated assays—a latex immunoturbidimetric assay (LIA) that evaluates competitive inhibition of a HIT‐like monoclonal antibody and a chemiluminescence immunoassay (CLIA) for detecting anti‐PF4/heparin IgG—optimizes diagnostic sensitivity while also yielding good specificity, particularly at high assay reactivities. Patients/Methods We determined operating characteristics using combined LIA/CLIA results from a HIT observational trial (n = 430; derivation cohort) and 147 consecutive patients with HIT (n = 147; supplementary derivation cohort). We also evaluated 678 consecutive samples referred for HIT testing (replication cohort). LIA/CLIA reactivities were scored individually as “negative” ( |
| Databáze: | OpenAIRE |
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