Changes in diaphragm structure following prolonged mechanical ventilation in piglets
| Autor: | L. Edström, T. Ansved, Lars Eriksson, Helena Stibler, Peter J. Radell |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
medicine.medical_specialty
Time Factors Swine medicine.medical_treatment Diaphragm Muscle Fibers Skeletal Inflammation Statistics Nonparametric Internal medicine Myosin Medical Illustration Respiratory muscle Image Processing Computer-Assisted Medicine Animals Myopathy Mechanical ventilation business.industry General Medicine Respiration Artificial Diaphragm (structural system) Respiratory Function Tests Microscopy Electron Anesthesiology and Pain Medicine Endocrinology Anesthesia Models Animal Breathing Electrophoresis Polyacrylamide Gel medicine.symptom business Myofibril |
| Zdroj: | Acta anaesthesiologica Scandinavica. 48(4) |
| ISSN: | 0001-5172 |
| Popis: | Background: Prolonged mechanical ventilation and inactivity negatively affect muscle function. The mechanisms for this dysfunction are unclear and clinical studies of respiratory muscle are difficult to carry out. An animal model simulating the critical care environment was used to investigate the effects of 5 days' mechanical ventilation and diaphragm inactivity on diaphragm muscle morphology. Methods: Twelve 2–4-month-old piglets weighing 23–30 kg were studied. Seven animals received controlled mechanical ventilation and sedation such that spontaneous breathing efforts were inhibited over 5 days. Five control animals were ventilated for only 4–6 h following surgical preparation. Diaphragm biopsies were obtained from the left costal region at the end of all experiments. Results: Morphometric, morphologic, electron microscopic and enzyme histochemical examination of costal diaphragm biopsies was carried out. Contractile properties were studied over 5 days and the results have been previously reported. Cross-sectional area of alI fiber types was increased compared with controls. The proportion of type IIb/x fibers increased following inactivity (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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