The antimicrobial effects of Citrus limonum and Citrus aurantium essential oils on multi-species biofilms
| Autor: | Antonio Olavo Cardoso Jorge, Cristiane Aparecida Pereira, Fernanda Bispo Reis Di Iorio, Sarah Almeida Coelho Oliveira, Jessica Rabelo Mina Zambrana |
|---|---|
| Přispěvatelé: | Universidade Estadual Paulista (Unesp), Universidade Federal de São Paulo (UNIFESP) |
| Rok vydání: | 2014 |
| Předmět: |
Citrus
Time Factors food.ingredient Serial dilution Surface Properties Acrylic Resins Colony Count Microbial Sodium Chloride Enterococcus faecalis Microbiology chemistry.chemical_compound food Anti-Infective Agents Candida albicans Escherichia coli medicine Plant Oils Agar General Materials Science Food science Citrus aurantium biology Chlorhexidine Biofilm Reproducibility of Results biology.organism_classification Antimicrobial lcsh:RK1-715 chemistry lcsh:Dentistry Biofilms Sodium hypochlorite Citrus limonum medicine.drug |
| Zdroj: | Brazilian Oral Research, Volume: 28, Issue: 1, Pages: 22-27, Published: 07 OCT 2013 Brazilian Oral Research v.28 n.1 2014 Brazilian Oral Research Sociedade Brasileira de Pesquisa Odontológica (SBPqO) instacron:SBPQO SciELO Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP Brazilian Oral Research, Vol 28, Iss 1, Pp 22-27 (2014) |
| ISSN: | 1806-8324 |
| DOI: | 10.1590/s1806-83242013005000024 |
| Popis: | Made available in DSpace on 2014-09-30T18:18:33Z (GMT). No. of bitstreams: 0 Previous issue date: 2014-01-01Bitstream added on 2014-10-01T14:03:18Z : No. of bitstreams: 1 S1806-83242014000100217.pdf: 1782439 bytes, checksum: c88bb6a3d5d1da890db5341dff4ec33a (MD5) The aim of this study was to evaluate the effects of Citrus limonum and Citrus aurantium essential oils (EOs) compared to 0.2% chlorhexidine (CHX) and 1% sodium hypochlorite (NaOCl) on multi-species biofilms formed by Candida albicans, Enterococcus faecalis and Escherichia coli. The biofilms were grown in acrylic disks immersed in broth, inoculated with microbial suspension (106 cells/mL) and incubated at 37°C / 48 h. After the biofilms were formed, they were exposed for 5 minutes to the solutions (n = 10): C. aurantium EO, C. limonum EO, 0.2% CHX, 1% NaOCl or sterile saline solution [0.9% sodium chloride (NaCl)]. Next, the discs were placed in sterile 0.9% NaCl and sonicated to disperse the biofilms. Tenfold serial dilutions were performed and the aliquots were seeded onto selective agar and incubated at 37°C / 48 h. Next, the number of colony-forming units per milliliter was counted and analyzed statistically (Tukey test, p ≤ 0.05). C. aurantium EO and NaOCl inhibited the growth of all microorganisms in multi-species biofilms. C. limonum EO promoted a 100% reduction of C. albicans and E. coli, and 49.3% of E. faecalis. CHX was less effective against C. albicans and E. coli, yielding a reduction of 68.8% and 86.7%, respectively. However, the reduction of E. faecalis using CHX (81.7%) was greater than that obtained using C. limonum EO. Both Citrus limonum and Citrus aurantium EOs are effective in controlling multi-species biofilms; the microbial reductions achieved by EOs were not only similar to those of NaOCl, but even higher than those achieved by CHX, in some cases. Universidade Estadual Paulista Institute of Science and Technology Department of Biosciences and Oral Diagnosis Universidade Federal de São Paulo Institute of Environmental, Chemical and Pharmaceutics Sciences Department of Earth and Exact Sciences Universidade Estadual Paulista Institute of Science and Technology Department of Biosciences and Oral Diagnosis |
| Databáze: | OpenAIRE |
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