Identification of COL1A1 as an invasion‑related gene in malignant astrocytoma
Autor: | Yue Wang, Fa‑Ping Wan, Xin-Feng Liu, Yue Gao, Qi Li, Yue Wu, Ayanlaja Abiola Abdulrahman, Li Li, Shen Sun, Guang‑Quan Ji, Dianshuai Gao, Jin Gao, Yun-Qing Li |
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Rok vydání: | 2018 |
Předmět: |
0301 basic medicine
Cancer Research malignant astrocytoma extracellular matrix Astrocytoma Collagen Type I 03 medical and health sciences Cell Line Tumor Databases Genetic laminin subunit γ1 Humans Gene Regulatory Networks Neoplasm Invasiveness KEGG Gene Regulation of gene expression Oncogene biology Brain Neoplasms Activator (genetics) Gene Expression Profiling Articles bioinformatics Prognosis invasion Survival Analysis Up-Regulation Collagen Type I alpha 1 Chain Gene Expression Regulation Neoplastic Gene expression profiling Fibronectin 030104 developmental biology Oncology Cancer research biology.protein Signal transduction collagen type I α1 chain Signal Transduction |
Zdroj: | International Journal of Oncology |
ISSN: | 1791-2423 1019-6439 |
Popis: | Malignant astrocytoma (MA) is the most common and severe type of brain tumor. A greater understanding of the underlying mechanisms responsible for the development of MA would be beneficial for the development of targeted molecular therapies. In the present study, the upregulated differentially expressed genes (DEGs) in MA were obtained from the Gene Expression Omnibus database using R/Bioconductor software. DEGs in different World Health Organization classifications were compared using the Venny tool and 15 genes, including collagen type I α1 chain (COL1A1) and laminin subunit γ1 (LAMC1), were revealed to be involved in the malignant progression of MA. In addition, the upregulated DEGs in MA were evaluated using functional annotations of Gene Ontology and Kyoto Encyclopedia of Genes and Genomes with the Database for Annotation, Visualization, and Integrated Discovery tool. The results indicated that invasion-associated enrichment was observed in 'extracellular matrix' (ECM), 'cell adhesion' and 'phosphoinositide 3-kinase-protein kinase B signaling pathway'. Subsequently, the analysis of the protein-protein interactions was performed using STRING and Cytoscape software, which revealed that the ECM component was the invasion-associated module and its corresponding genes included COL1A1, LAMC1 and fibronectin 1. Finally, survival Kaplan-Meier estimate was conducted using cBioportal online, which demonstrated that COL1A1 expression affected the survival of and recurrence in patients with MA. Moreover, the results of in vitro Transwell assay and western blot analysis revealed that the depleted levels of COL1A1 also decreased the expression of several proteins associated with cell invasion, including phosphorylated-signal transducer and activator of transcription 3, matrix metalloproteinase (MMP)-2, MMP-9 and nuclear factor-κB. On the whole, the present study identified the invasion-related target genes and the associated potential pathways in MA. The results indicated that COL1A1 may be a candidate biomarker for the prognosis and treatment of MA. |
Databáze: | OpenAIRE |
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