Antioxidant and anti-inflammatory effects of N-acetylcysteine against malathion-induced liver damages and immunotoxicity in rats

Autor: Jaouida Abdelmoula, Kahena Bouzid, Alya Annabi, Ines Bini Dhouib, Mohamed Montassar Lasram, Nadia Belhadjhmida, Saloua El Fazaa, Aicha Jrad Lamine, Najoua Gharbi, Malika Ben Ahmed
Rok vydání: 2014
Předmět:
Male
Insecticides
Anti-Inflammatory Agents
Apoptosis
Pharmacology
medicine.disease_cause
Antioxidants
Acetylcysteine
Lipid peroxidation
chemistry.chemical_compound
Leukocytes
Lymphocytes
General Pharmacology
Toxicology and Pharmaceutics
Liver injury
biology
Reverse Transcriptase Polymerase Chain Reaction
General Medicine
Free Radical Scavengers
Flow Cytometry
Liver
Anesthesia
Toxicity
Malathion
Cytokines
Chemical and Drug Induced Liver Injury
medicine.drug
Blotting
Western
Aspartate transaminase
Real-Time Polymerase Chain Reaction
General Biochemistry
Genetics and Molecular Biology
medicine
Animals
RNA
Messenger
Rats
Wistar
Cell Proliferation
Peroxidase
Inflammation
business.industry
medicine.disease
Rats
Oxidative Stress
chemistry
Alanine transaminase
biology.protein
Lipid Peroxidation
business
Oxidative stress
Biomarkers
Zdroj: Life sciences. 107(1-2)
ISSN: 1879-0631
Popis: Aims Occupational exposure to organophosphate pesticides is becoming a common and increasingly alarming world-wide phenomenon. The present study is designed to investigate the preventive effect of N-acetylcysteine on malathion-induced hepatic injury and inflammation in rats. Main methods Adult male Wistar rats of body weight 200–230 g were used for the study. Malathion (200 mg/kg b.w./day) was administered to rats by oral intubation and N-acetylcysteine (2 g/l) in drinking water for 28 days. Rats were sacrificed on the 28th day, 2 h after the last administration. Markers of liver injury (aspartate transaminase, alanine transaminase, alkaline phosphatase and lactate desyhdogenase), inflammation (leukocyte counts, myeloperoxidase, immunophenotyping of CD4 + and CD8 + , interleukin-1β, interleukin-6 and interferon-γ expression) and oxidative stress (lipid peroxidation, reduced glutathione and antioxidant status) were assessed. Key findings Malathion induced an increase in activities of hepatocellular enzymes in plasma, lipid peroxidation index, CD3 + /CD4 + and CD3 + /CD4 + percent and pro-inflammatory cytokines, when decreased antioxidant status in liver was noted. When malathion-treated rats were compared to NAC supplemented rats, leukocytosis, T cell count and IL-1β, IL-6, INF-γ expression were reduced. Furthermore, NAC restored liver enzyme activities and oxidative stress markers. Significance Malathion induces hepatotoxicity, oxidative stress and liver inflammation. N-acetylcysteine showed therapeutic effects against malathion toxicity.
Databáze: OpenAIRE
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