Metabolism of Orthotopic Mouse Brain Tumor Models
Autor: | Marvin D. Nelson, Stefan Bluml, Jonathan L. Finlay, Goar Smbatyan, Anat Erdreich-Epstein, Ignacio Gonzalez-Gomez, Michael Rosol, Elizabeth Melendez, Mark D. Krieger, C. Patrick Reynolds, Jingying Xu, Ira Harutyunyan |
---|---|
Jazyk: | angličtina |
Rok vydání: | 2009 |
Předmět: |
Male
Choroid Plexus Neoplasms Pathology medicine.medical_specialty Magnetic Resonance Spectroscopy lcsh:Medical technology Transplantation Heterologous Biomedical Engineering Brain tumor Mice Nude Heterologous Mice SCID Biology Creatine Mice chemistry.chemical_compound Mice Inbred NOD Tumor Cells Cultured medicine Animals Humans Choline Radiology Nuclear Medicine and imaging Cerebellar Neoplasms Child lcsh:QH301-705.5 Rhabdoid Tumor Medulloblastoma Brain Neoplasms Carcinoma Teratoma Infant Choroid plexus carcinoma Condensed Matter Physics medicine.disease Radiography Transplantation chemistry lcsh:Biology (General) lcsh:R855-855.5 Cell culture Child Preschool Molecular Medicine Female Biotechnology |
Zdroj: | Molecular Imaging, Vol 8 (2009) |
ISSN: | 1536-0121 |
Popis: | We used magnetic resonance spectroscopy to determine whether orthotopic mouse brain tumors grown as xenografts in immunocompromised mice either from human brain tumor cells implanted immediately after surgery or from cultured human tumor lines show metabolic profiles comparable to those of the original tumors. Using a 7 T scanner, spectra were acquired from mice with a human atypical teratoid/rhabdoid tumor (AT/RT) either implanted directly from the surgical specimen or first grown in culture, directly implanted choroid plexus carcinoma (CPC), and two medulloblastoma cell lines. The results were compared with spectra from these same tumors or tumor types in patients and with controls. Metabolic variability of tumors from a single cell line was also evaluated using the medulloblastoma lines. The main metabolic features of human tumors were qualitatively replicated in xenografts. AT/RTs in mice exhibited choline, creatine, and myo -inositol levels comparable to those observed in the patient. As in patients, choline was prominent in experimental CPC. Tumors from a single cell line were comparable. Significant correlations were found with key metabolites in humans and mice; however, differences including lower lipids in the implanted AT/RTs than in patient spectra and taurine observed in all animal spectra were also noted. The causes of these dissimilarities warrant further investigation. |
Databáze: | OpenAIRE |
Externí odkaz: |