Differential placental expression profile of human Growth Hormone/Chorionic Somatomammotropin genes in pregnancies with pre-eclampsia and gestational diabetes mellitus

Autor: Pille Vaas, Kristiina Rull, Maris Laan, Jaana Männik, Pille Teesalu
Rok vydání: 2012
Předmět:
Placenta
Gene Expression
Fetal growth
Somatomammotropin
Gestational diabetes mellitus
Biochemistry
0302 clinical medicine
Endocrinology
Pre-Eclampsia
Pregnancy
Gene expression
Protein Isoforms
reproductive and urinary physiology
0303 health sciences
Alternative mRNA transcripts
Placental expression
female genital diseases and pregnancy complications
Gestational diabetes
030220 oncology & carcinogenesis
Pituitary Gland
embryonic structures
Female
Adult
medicine.medical_specialty
Biology
Article
03 medical and health sciences
Internal medicine
Diabetes mellitus
medicine
Humans
RNA
Messenger
Molecular Biology
030304 developmental biology
Eclampsia
Gene Expression Profiling
Intron
Infant
Newborn
hGH/CSH genes
medicine.disease
Placental Lactogen
Introns
Gene expression profiling
Alternative Splicing
Diabetes
Gestational
Case-Control Studies
Growth Hormone
Placental Hormones
Zdroj: Molecular and Cellular Endocrinology
ISSN: 0303-7207
DOI: 10.1016/j.mce.2012.02.009
Popis: Highlights ► A trend for lower level of all placental hGH/CSH transcripts in preeclampsia. ► Reduced GH2-2/CSH1-2 transcripts retaining intron 4 only in preeclampsia without SGA. ► Increased level of placental GH2 mRNA in gestational diabetes with LGA newborns. ► hGH/CSH genes exhibit pleiotropic effects on fetal growth and maternal metabolism.
The human GH/CSH cluster consisting of one pituitary-expressed (GH1) and four placenta-expressed loci has been implicated in maternal metabolic adaptation to pregnancy, regulation of intrauterine and postnatal growth. We investigated how the mRNA expression profile of placental GH2, CSH1 and CSH2 genes and their alternative transcripts correlates with maternal pre-eclampsia (PE) and/or gestational diabetes mellitus (GD). The expression of studied genes in PE placentas (n = 17) compared to controls (n = 17) exhibited a trend for reduced transcript levels. The alternative transcripts retaining intron 4, GH2-2 and CSH1-2 showed significantly reduced expression in PE cases without growth restriction (P = 0.007, P = 0.008, respectively). In maternal GD (n = 23), a tendency of differential expression was detected only for the GH2 gene and in pregnancies with large-for-gestational-age newborns. Our results, together with those reported by others, are consistent with a pleiotropic effect of placental hGH/CSH genes at the maternal-fetal interface relating to the regulation of fetal growth and the risk of affected maternal metabolism.
Databáze: OpenAIRE
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