Characterization of New Oligosaccharides Obtained by An Enzymatic Cleavage of the Exopolysaccharide Produced by the Deep-Sea Bacterium Alteromonas infernus Using its Cell Extract
| Autor: | Hélène Rogniaux, Katy Akoumany, David Ropartz, Sylvia Colliec-Jouault, Christine Delbarre-Ladrat, Laëtitia Marchand, Mathieu Fanuel, Muriel Pipelier, Corinne Sinquin, Agata Zykwinska |
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| Přispěvatelé: | Laboratoire Ecosystèmes Microbiens et Molécules Marines pour les Biotechnologies [IFREMER, Nantes], Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Chimie Et Interdisciplinarité : Synthèse, Analyse, Modélisation (CEISAM), Université de Nantes - UFR des Sciences et des Techniques (UN UFR ST), Université de Nantes (UN)-Université de Nantes (UN)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Unité de recherche sur les Biopolymères, Interactions Assemblages (BIA), Institut National de la Recherche Agronomique (INRA) |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
glycosaminoglycan-mimetic
Pharmaceutical Science Polysaccharide Cleavage (embryo) Alteromonas infernus 01 natural sciences Analytical Chemistry lcsh:QD241-441 Glycosaminoglycan 03 medical and health sciences lcsh:Organic chemistry deep-sea bacterium Drug Discovery [CHIM]Chemical Sciences structural analysis Physical and Theoretical Chemistry Lyase activity 030304 developmental biology mass spectrometry chemistry.chemical_classification 0303 health sciences biology 010405 organic chemistry Depolymerization Chemistry exopolysaccharides Organic Chemistry Oligosaccharide biology.organism_classification 0104 chemical sciences Enzyme Biochemistry 13. Climate action Chemistry (miscellaneous) wild-type strain enzymatic depolymerization Molecular Medicine Bacteria |
| Zdroj: | Molecules (1420-3049) (MDPI AG), 2019-10, Vol. 24, N. 19, P. 3441 (15p.) Molecules Molecules, MDPI, 2019, 24 (19), pp.1-15. ⟨10.3390/molecules24193441⟩ Molecules, Vol 24, Iss 19, p 3441 (2019) Volume 24 Issue 19 |
| ISSN: | 1420-3049 |
| Popis: | Bacteria from deep-sea hydrothermal vents constitute an attractive source of bioactive molecules. In particular, exopolysaccharides (EPS) produced by these bacteria become a renewable source of both biocompatible and biodegradable molecules. The low molecular weight (LMW) derivatives of the GY785 EPS produced by the deep-sea hydrothermal vent strain Alteromonas infernus have previously displayed some biological properties, similar to those of glycosaminoglycans (GAG), explored in cancer and tissue engineering. These GAG-mimetic derivatives are obtained through a free radical depolymerization process, which could, however, affect their structural integrity. In a previous study, we have shown that A. infernus produces depolymerizing enzymes active on its own EPS. In the present study, an enzymatic reaction was optimized to generate LMW derivatives of the GY785 EPS, which could advantageously replace the present bioactive derivatives obtained by a chemical process. Analysis by mass spectrometry of the oligosaccharide fractions released after enzymatic treatment revealed that mainly a lyase activity was responsible for the polysaccharide depolymerization. The repeating unit of the GY785 EPS produced by enzyme cleavage was then fully characterized. |
| Databáze: | OpenAIRE |
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