Clonal growth of hamster free alveolar cells in soft agar
| Autor: | C. Kuhn, T. T. Kuo, Hsiu-San Lin |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1975 |
| Předmět: |
Male
Pathology medicine.medical_specialty Plating efficiency food.ingredient Cell division Immunology Hamster Bone Marrow Cells Biology In Vitro Techniques Alveolar cells Mice food Colony-Stimulating Factors Soft agar Cricetinae medicine Baby hamster kidney cell Immunology and Allergy Agar Animals Phagocytes Macrophages Articles respiratory system Colony-stimulating factor Molecular biology Clone Cells Rats Pulmonary Alveoli medicine.anatomical_structure Cell Division |
| Zdroj: | The Journal of Experimental Medicine |
| ISSN: | 1540-9538 0022-1007 |
| Popis: | Free alveolar cells obtained from healthy unstimulated hamsters were tested for their ability to form colonies in soft agar. Every bronchial washing so far tested contained colon-forming cells. The average plating efficiency was 8.1% (2.4-18.3%). Alveolar colony-forming cells were characterized by having a long initial lag period (4-8 days) and only mononuclear phagocytes were found in the colony. Medium conditioned by baby hamster kidney cells or other cells was required for the initiation and maintenance of their growth. Alveolar cells from normal mice and rats also formed colonies under appropriate culture conditions. |
| Databáze: | OpenAIRE |
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