Dietary omega 3 fatty acids and cholesterol modify enterocyte microsomal membrane phospholipids, cholesterol content and phospholipid enzyme activities in diabetic rats
| Autor: | K. Doring, E. Wierzbicki, M. Tavernini, Michael T. Clandinin, Abr Thomson, Monika Keelan |
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| Rok vydání: | 1994 |
| Předmět: |
medicine.medical_specialty
Phospholipid Biology Biochemistry Diabetes Mellitus Experimental Cholesterol Dietary chemistry.chemical_compound Membrane Lipids Dietary Fats Unsaturated Internal medicine Phosphatidylcholine Microsomes Fatty Acids Omega-3 medicine Animals Intestinal Mucosa Rats Wistar Phospholipids chemistry.chemical_classification Phosphatidylethanolamine Cholesterol Organic Chemistry Fatty acid Cell Biology Fish oil Rats Intestines Endocrinology chemistry Phosphatidylethanolamine N-methyltransferase lipids (amino acids peptides and proteins) Female Polyunsaturated fatty acid |
| Zdroj: | Lipids. 29(12) |
| ISSN: | 0024-4201 |
| Popis: | Diabetes-associated changes in intestinal uptake of nutrients are modified by isocaloric variations in the type of dietary lipids, and are associated with alterations in the phospholipid and fatty acyl content of the intestinal brush border membrane. The present study was designed to test the hypothesis that diet- and diabetes-associated changes in enterocyte microsomal membrane phospholipids are due to variations in the activity of two phospholipid metabolizing enzymes, 1,2-diacylglycerol: CDP choline cholinephosphotransferase (CPT) and phosphatidylethanolamine methyltransferase (PEMT). Adult female Wistar rats were fed one of four semisynthetic diets—beef tallow low in cholesterol (BT), beef tallow high in cholesterol (BTC), fish oil low in cholesterol (FO) or fish oil high in cholesterol. In half of the animals, diabetes mellitus was produced by injection of streptozotocin. Jejunal and ileal enterocyte microsomes (EMM) were isolated and analyzed for cholesterol and phospholipids, as well as for CPT and PEMT activities. In control animals, feeding FO reduced EMM total phospholipids including phosphatidylcholine (PC), phosphatidylethanolamine (PE) and phosphatidylinositol. Feeding FO resulted in a greater than 95% reduction in the activity of CPT. Diabetes was associated with increased jejunal EMM total phospholipids including sphingomyelin (SM) and PE, without associated changes in CPT or PEMT. Dietary cholesterol supplementation did not effect EMM total cholesterol or phospholipid composition in control rats fed BT or FO, but was associated with an increase in EMM cholesterol in diabetic rats fed BT or FO. A decrease in total phospho-lipids due to a decline in SM, PC and PE in diabetic rats fed FO was not associated with changes in the activities of CPT or PEMT in EMM. Thus (i) enterocyte microsomal membrane cholesterol and phospholipid contents are influenced by diabetes, dietary cholesterol and the type of fatty acid in the diet, and (ii) changes in phospholipid composition are not fully explained by alterations in the activities of CPT and PEMT. |
| Databáze: | OpenAIRE |
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