Direct genotypic characterization of Toxoplasma gondii strains associated with congenital toxoplasmosis in Tunisia (North Africa)
| Autor: | Karim Aoun, O. Souissi, Sonia Boughattas, Rym Ben-Abdallah, E. Siala, Aïda Bouratbine |
|---|---|
| Přispěvatelé: | Institut Pasteur de Tunis, Réseau International des Instituts Pasteur (RIIP) |
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Genetic Markers
Tunisia Genotype MESH: Genetic Markers Genetic analysis Polymerase Chain Reaction Toxoplasmosis Congenital MESH: Pregnancy Complications Parasitic law.invention MESH: Genotype MESH: Pregnancy law Polymorphism (computer science) Pregnancy Virology parasitic diseases MESH: Polymorphism Genetic medicine Animals Humans MESH: Animals Polymerase chain reaction Polymorphism Genetic MESH: Humans biology MESH: Polymorphism Restriction Fragment Length MESH: Toxoplasma Toxoplasma gondii MESH: Polymerase Chain Reaction Articles biology.organism_classification medicine.disease MESH: Toxoplasmosis Congenital Toxoplasmosis Infectious Diseases [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology Genetic marker Pregnancy Complications Parasitic Parasitology Female Restriction fragment length polymorphism MESH: Tunisia Toxoplasma MESH: Female Polymorphism Restriction Fragment Length |
| Zdroj: | American Journal of Tropical Medicine and Hygiene American Journal of Tropical Medicine and Hygiene, American Society of Tropical Medicine and Hygiene, 2010, 82 (6), pp.1041-6. ⟨10.4269/ajtmh.2010.09-0691⟩ |
| ISSN: | 0002-9637 |
| DOI: | 10.4269/ajtmh.2010.09-0691⟩ |
| Popis: | International audience; Here, we determined the Toxoplasma gondii genotype in amniotic fluid, placenta, and cerebrospinal fluid samples from 14 congenital toxoplasmosis cases in Tunisia, North Africa. Direct genotypic characterization of T. gondii strains was performed by polymerase chain reaction (PCR) amplification of six genetic markers (3'SAG2, 5' SAG2, SAG3, BTUB, GRA6, and APICO) and thereafter, was analyzed by restriction fragment-length polymorphism (RFLP). Samples were sequenced to resolve strain type whenever there were unclear enzyme digestion results. Multilocus analysis revealed that only one specimen harbored the type I allele in all studied loci, whereas the 13 others gave mixed genotype results with different alleles at different markers. Seven specimens produced RFLP profile of the recombinant strains I/III, and three produced a profile of I/II recombinant strains. The last three specimens produced complex digestion patterns. In these cases, sequence analysis revealed double peaks at known polymorphic sites, indicating the presence of multiple alleles. |
| Databáze: | OpenAIRE |
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