COL1A2 is a TBX3 target that mediates its impact on fibrosarcoma and chondrosarcoma cell migration
| Autor: | Rehana Omar, Sharon Prince, Luiz F. Zerbini, Aretha Cooper, Hapiloe Mabaruti Maranyane |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
musculoskeletal diseases
0301 basic medicine Transcriptional Activation Cancer Research Fibrosarcoma Chondrosarcoma AKT1 Bone Neoplasms macromolecular substances Biology medicine.disease_cause Collagen Type I Cell Line 03 medical and health sciences 0302 clinical medicine Cell Movement Cell Line Tumor medicine Humans Phosphorylation Promoter Regions Genetic Protein kinase B Transcription factor integumentary system Cell migration Fibroblasts medicine.disease Cell biology 030104 developmental biology Oncology 030220 oncology & carcinogenesis Carcinogenesis T-Box Domain Proteins Chromatin immunoprecipitation Proto-Oncogene Proteins c-akt Signal Transduction |
| Zdroj: | Cancer letters. 459 |
| ISSN: | 1872-7980 |
| Popis: | The developmentally important T-box transcription factor TBX3, is overexpressed in several cancers and contributes to tumorigenesis as either a tumour promoter or tumour suppressor. For example, TBX3 promotes cell proliferation, migration and invasion of chondrosarcoma cells but inhibits these processes in fibrosarcoma cells. This suggests that the cellular context influences TBX3 oncogenic functions, but the mechanism(s) involved has not been elucidated. COL1A2 encodes type I collagen and, like TBX3, plays important roles during embryogenesis and can act as either oncogene or tumour suppressor. Here we explore the possibility that COL1A2 may be a TBX3 target gene responsible for mediating its opposing oncogenic roles in chondrosarcoma and fibrosarcoma cells. Results from qRT-PCR, western blotting, luciferase reporter and chromatin immunoprecipitation assays show that TBX3 binds and activates the COL1A2 promoter. Furthermore, we show that TBX3 levels are regulated by AKT1 and that pseudo-phosphorylation of TBX3 at an AKT consensus serine site, enhances its ability to activate COL1A2. Importantly, we demonstrate that COL1A2 mediates the pro- and anti-migratory effects of TBX3 in chondrosarcoma and fibrosarcoma cells respectively. Our data reveal that the AKT1/TBX3/COL1A2 axis plays an important role in sarcomagenesis. |
| Databáze: | OpenAIRE |
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