Improvement of the identification of staphylococci isolated from bovine mammary infections using molecular methods
| Autor: | Jerome Etienne, V. Guérin-Faublée, Jean Freney, Michèle Bes, Hélène Meugnier |
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| Rok vydání: | 2000 |
| Předmět: |
DNA
Bacterial Micrococcaceae Genotype Staphylococcus Colony Count Microbial Subspecies medicine.disease_cause DNA Ribosomal Polymerase Chain Reaction Microbiology law.invention Mammary Glands Animal law Polymorphism (computer science) medicine Animals Mastitis Bovine Ribosomal DNA Polymerase chain reaction Genetics Polymorphism Genetic General Veterinary biology General Medicine Staphylococcal Infections biology.organism_classification Milk Phenotype Cattle Female Bacteria |
| Zdroj: | Veterinary Microbiology. 71:287-294 |
| ISSN: | 0378-1135 |
| DOI: | 10.1016/s0378-1135(99)00163-7 |
| Popis: | Fifty-six Staphylococcus strains isolated from cases of bovine mammary infections were identified by using phenotypic and genotypic methods. Twenty-eight strains (50%) were identified at the species level according to their phenotypic characteristics, whereas the remaining 28 strains presented atypical or unreliable profiles. A combination of phenotypic and genotypic methods allowed the 56 strains studied to be classified. Internal transcribed spacer-polymerase chain reaction (ITS-PCR) based on the polymorphism of the 16S-23S rDNA spacer region appeared as a rapid and reliable method for the classification of bovine staphylococcal isolates at the species and subspecies levels. |
| Databáze: | OpenAIRE |
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