Novel approaches to characterize age-related remodelling of the dermal-epidermal junction in 2D, 3D and in vivo
Autor: | Marie Cherel, Pierre Seroul, Rachel E.B. Watson, Michael J. Sherratt, Christopher E.M. Griffiths, Rainer Voegeli, A. V. Rawlings, Victoria L. Newton, Robert S. Bradley |
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Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
Reflectance confocal microscopy Adult Male Aging Adolescent Dermatology Biology 030207 dermatology & venereal diseases 03 medical and health sciences Young Adult 0302 clinical medicine Imaging Three-Dimensional Dermis In vivo Age related medicine Humans Dermoepidermal junction X-ray computed tomography Photodamage Microscopy Confocal integumentary system Rete ridge Anatomy Skin Aging 030104 developmental biology Dermal papillae medicine.anatomical_structure Epidermal Cells Skin ageing Dermal-epidermal junction Tomography Epidermis Dermal papilla Tomography X-Ray Computed |
Zdroj: | Newton, V L, Bradley, R S, Seroul, P, Cherel, M, Griffiths, C E M, Rawlings, A V, Voegeli, R, Watson, R E B & Sherratt, M J 2016, ' Novel approaches to characterize age-related remodelling of the dermal-epidermal junction in 2D, 3D and in vivo ', Skin Research and Technology . https://doi.org/10.1111/srt.12312 |
ISSN: | 1600-0846 |
Popis: | BACKGROUND/PURPOSE: The dermal-epidermal junction (DEJ) forms epidermal protrusions down into the dermis (rete ridges) and dermal projections up into the epidermis (dermal papillae). Usually visualized in two-dimensions (2D), our knowledge of how the DEJ changes with ageing is limited. We aimed to characterize how this structure exists in 3D and changes with age.METHODS: Photoprotected and photoexposed skin were imaged using reflectance confocal microscopy (RCM) in young and aged individuals. Biopsies of the imaged areas were processed for histological sectioning and for imaging using micro-computed X-ray tomography (microCT).RESULTS: Images obtained from RCM and microCT were used to 3D reconstruct the DEJ. DEJ heights obtained from microCT images showed strong correlation with histology-measured heights. We proposed a novel definition of rete ridges (RRm ) and dermal papillae (DPm ), which allowed easier automated measurement of reduced DPm and RRm volumes in aged skin from microCT reconstructions. An algorithm to map DPm connectivity showed reduced lengths of DPm branches with age.CONCLUSION: Three-dimensional images illustrated the complex topography of the DEJ and highlighted the distinct morphology of dermal papillae compared with rete ridges, which is not evident when evaluating 2D sections. Ex vivo imaging was more successful in differentiating DEJ architecture with respect to age. |
Databáze: | OpenAIRE |
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