Pre- and postmenopausal women have different core urinary microbiota

Autor:	Karin Glavind, Annemarie Brusen Villadsen, Louise Thomsen Schmidt Arenholt, Nadia Ammitzbøll, Caspar Bundgaard-Nielsen, Ann Maria Jensen, Benedikt Paul Josef Bau, Peter Derek Christian Leutscher, Søren Hagstrøm, Suzette Sørensen
Jazyk:	angličtina
Rok vydání:	2021
Předmět:	0301 basic medicine Adult Urinary system Bladder Science Physiology Urine Disease Microbiology Article 03 medical and health sciences 0302 clinical medicine Lactobacillus Prevotella Medicine Humans 030219 obstetrics & reproductive medicine Multidisciplinary Postmenopausal women biology Bacteria business.industry Microbiota Middle Aged biology.organism_classification Pathophysiology Postmenopause 030104 developmental biology Premenopause Female business Hormone
Zdroj:	Scientific Reports, Vol 11, Iss 1, Pp 1-10 (2021) Scientific Reports Ammitzbøll, N, Bau, B P J, Bundgaard-Nielsen, C, Villadsen, A B, Jensen, A M, Leutscher, P D C, Glavind, K, Hagstrøm, S, Arenholt, L T S & Sørensen, S 2021, ' Pre-and postmenopausal women have different core urinary microbiota ', Scientific Reports, vol. 11, 2212 . https://doi.org/10.1038/s41598-021-81790-8
ISSN:	2045-2322
DOI:	10.1038/s41598-021-81790-8
Popis:	Recent studies suggest that alterations in the female urinary microbiota is associated to development of bladder disease. However, the normal microbiota composition and variation in healthy women are poorly described. Moreover, the effects of hormonal changes on microbiota during menopause is not well understood. The aim of our study was to investigate the urinary microbiota in healthy pre- and postmenopausal women without urinary tract symptoms. Microbiota composition in catheterized urine samples was mapped using 16S rRNA gene sequencing. In total, 41 premenopausal and 42 postmenopausal women were initially included. Samples with first PCR amplification concentration below level of the negative control were excluded, resulting in 34 premenopausal and 20 postmenopausal women included in data analysis. Urine from postmenopausal women showed significantly higher alpha diversity compared to premenopausal women. Lactobacillus was the most abundant bacteria in both groups, however the relative abundance of Lactobacillus accounted for 77.8% in premenopausal versus 42.0% in postmenopausal women. In conclusion, urine from premenopausal mostly presented with Lactobacillus dominated urotypes, whereas urine from postmenopausal women presented a more diverse urinary microbiota with higher abundance of the genera Gardnerella and Prevotella. The clinical and pathophysiological implications of this difference remain to be elucidated.
Databáze:	OpenAIRE
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