Characterization of tissue biomechanics and mechanical signaling in uterine leiomyoma
| Autor: | Casey L. Korecki, J.M. Norian, Rocky S. Tuan, James H. Segars, C.M. Owen, Minnie Malik, William H. Catherino, Juan M. Taboas |
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| Rok vydání: | 2012 |
| Předmět: |
Adult
medicine.medical_specialty RHOA A Kinase Anchor Proteins Article Minor Histocompatibility Antigens Extracellular matrix Elastic Modulus Proto-Oncogene Proteins Internal medicine medicine Humans Mechanotransduction neoplasms Molecular Biology Rho-associated protein kinase Cells Cultured rho-Associated Kinases Uterine leiomyoma Leiomyoma biology Chemistry Uterus Myometrium Middle Aged musculoskeletal system Actin cytoskeleton medicine.disease Elasticity female genital diseases and pregnancy complications Biomechanical Phenomena Extracellular Matrix Cell biology body regions surgical procedures operative Endocrinology Uterine Neoplasms biology.protein Female Stress Mechanical rhoA GTP-Binding Protein Signal Transduction |
| Zdroj: | Matrix Biology. 31:57-65 |
| ISSN: | 0945-053X |
| Popis: | Leiomyoma are common tumors arising within the uterus that feature excessive deposition of a stiff, disordered extracellular matrix (ECM). Mechanical stress is a critical determinant of excessive ECM deposition and increased mechanical stress has been shown to be involved in tumorigenesis. Here we tested the viscoelastic properties of leiomyoma and characterized dynamic and static mechanical signaling in leiomyoma cells using three approaches, including measurement of active RhoA. We found that the peak strain and pseudo-dynamic modulus of leiomyoma tissue was significantly increased relative to matched myometrium. In addition, leiomyoma cells demonstrated an attenuated response to applied cyclic uniaxial strain and to variation in substrate stiffness, relative to myometrial cells. However, on a flexible pronectin-coated silicone substrate, basal levels and lysophosphatidic acid-stimulated levels of activated RhoA were similar between leiomyoma and myometrial cells. In contrast, leiomyoma cells plated on a rigid polystyrene substrate had elevated levels of active RhoA, compared to myometrial cells. The results indicate that viscoelastic properties of the ECM of leiomyoma contribute significantly to the tumor’s inherent stiffness and that leiomyoma cells have an attenuated sensitivity to mechanical cues. The findings suggest there may be a fundamental alteration in the communication between the external mechanical environment (extracellular forces) and reorganization of the actin cytoskeleton mediated by RhoA in leiomyoma cells. Additional research will be needed to elucidate the mechanism(s) responsible for the attenuated mechanical signaling in leiomyoma cells. |
| Databáze: | OpenAIRE |
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