Anticancer potential of methanolic extracts from Pleurotus species on raji cells and antibacterial activity against Methicillin-Resistant Staphylococcus aureus
| Autor: | Muhammad Evy Prastiyanto, Rizal Maarif Rukmana, Yanuarita Tursinawati, Dewi Kunthy Saraswati, Endang Tri Wahyuni Maharani, Sri Darmawati |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Pleurotus Minimum bactericidal concentration biology Traditional medicine QH301-705.5 Chemistry Plant Science antibacterial activity anticancer mrsa mushrooms pleurotus biology.organism_classification medicine.disease_cause 01 natural sciences Terpenoid Minimum inhibitory concentration Staphylococcus aureus 010608 biotechnology medicine Animal Science and Zoology Pleurotus ostreatus Biology (General) Antibacterial activity Molecular Biology 010606 plant biology & botany Antibacterial agent |
| Zdroj: | Biodiversitas, Vol 21, Iss 12 (2020) |
| ISSN: | 2085-4722 1412-033X |
| DOI: | 10.13057/biodiv/d211221 |
| Popis: | Prastiyanto ME, Rukmana RM, Saraswati DK, Darmawati S, Maharani ETW, Tursinawati Y. 2020. Anticancer potential of methanolic extracts from Pleurotus species on raji cells and antibacterial activity against Methicillin-Resistant Staphylococcus aureus. Biodiversitas 21: 5644-5649. The aim of this work is to identify the potential effects of methanolic extracts from four species of the Pleurotus genus cultivated in Indonesia on nasopharynx cancer (Raji cell line), and to investigate their antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA). This study investigates four species members of Pleurotus (Pleurotus ostreatus, P. cystidiosus, P. flabellatus, and P. pulmonarius var. stechangii). Dry Samples were extracted with methanol to yield crude extracts. Cytotoxicity screening was conducted using MTT assay of dry extracts, while antibacterial activity was calculated based on the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) using Mueller–Hinton broth, via the microdilution method. Compounds were analyzed using thin-layer chromatography (TLC). P. flabellatus provided the highest yield of dry extract (20.8%) with the lowest value of IC50 (556.226 µg/mL) compared to the three other species investigated. Antibacterial activity was calculated as MIC and MBC values against MRSA by the P. flabellatus extract which reached 6.25 mg/mL and 250 mg/mL, respectively. The result of TLC of the dry extract of P. flabellatus revealed the presence of terpenoids. P. flabellatus has the potential to be developed as both an anti-cancer and an antibacterial agent, especially against Raji cells and MRSA strains. However, further in vivo research and discovery of the modes of action involved are still needed to shed light on these effects. Studies can provide new information about the benefits of Pleurotus as a source of natural anticancer and antibacterial compound. |
| Databáze: | OpenAIRE |
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