Effects of PAMAM dendrimers with various surface functional groups and multiple generations on cytotoxicity and neuronal differentiation using human neural progenitor cells
| Autor: | Hideko Sone, Zhenya Zhang, Seishiro Hirano, Tin-Tin Win-Shwe, Yang Zeng, Yoshika Kurokawa, Qin Zeng |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Dendrimers Time Factors Cell Survival Surface Properties Stereochemistry DNA damage Neurogenesis Apoptosis Receptor Tyrosine Kinase-like Orphan Receptors Toxicology medicine.disease_cause Transforming Growth Factor beta1 Structure-Activity Relationship 03 medical and health sciences Neural Stem Cells Dendrimer medicine Humans Viability assay Particle Size Cytotoxicity Cells Cultured Dose-Response Relationship Drug Molecular Structure Chemistry Retinoic Acid 4-Hydroxylase Neural stem cell Oxidative Stress 030104 developmental biology Gene Expression Regulation Biophysics Oxidative stress DNA Damage |
| Zdroj: | The Journal of Toxicological Sciences. 41:351-370 |
| ISSN: | 1880-3989 0388-1350 |
| Popis: | Polyamidoamine (PAMAM) dendrimers have potential for biological applications as delivery systems for genes, drugs, and imaging agents into the brain, but their developmental neurotoxicity remains unknown. We investigated the effects of PAMAM dendrimers with various surface functional groups and multiple generations on neuronal differentiation using human neural progenitor cells at an equal mass concentration. Only PAMAM dendrimers containing amine (NH2) surface groups at concentrations of 10 μg/mL significantly reduced cell viability and neuronal differentiation, compared with non-amine-terminated dendrimers. PAMAM-NH2 with generation (G)3, G4, G5 G6, and G7 significantly decreased cell viability and inhibited neuronal differentiation from a concentration of 5 μg/mL, but G0, G1, and G2 dendrimers did not have any effect at this concentration. Cytotoxicity indices of PAMAM-NH2 dendrimers at 10 μg/mL correlated well with the zeta potentials of the particles. Surface group density and particle number in unit volume is more important characteristic than particle size to influence cytotoxicity for positive changed dendrimers. PAMAM-50% C12 at 1 μg/mL altered the expression level of the oxidative stress-related genes, ROR1, CYP26A1, and TGFB1, which is a DNA damage response gene. Our results indicate that PAMAM dendrimer exposure may have a surface charge-dependent adverse effect on neuronal differentiation, and that the effect may be associated with oxidative stress and DNA damage during development of neural cells. |
| Databáze: | OpenAIRE |
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