Application of in vivo and ex vivo magnetic resonance imaging for evaluation of tranilast on neointima formation following balloon angioplasty of the rat carotid artery
| Autor: | Lynne V. Clark, Eliot H. Ohlstein, Anne M. Romanic, Rasesh D. Kapadia, Susanta K. Sarkar, Sudeep Chandra, Robert Gagnon |
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| Rok vydání: | 2000 |
| Předmět: |
Male
Neointima Pathology medicine.medical_specialty Physiology medicine.medical_treatment Tranilast Lumen (anatomy) Coronary Disease Catheterization Rats Sprague-Dawley Restenosis Recurrence In vivo Physiology (medical) Angioplasty Anti-Allergic Agents medicine Animals ortho-Aminobenzoates Angioplasty Balloon Coronary Analysis of Variance medicine.diagnostic_test business.industry Magnetic resonance imaging medicine.disease Magnetic Resonance Imaging Rats Disease Models Animal Carotid Arteries Collagen Tunica Intima Cardiology and Cardiovascular Medicine business Ex vivo medicine.drug |
| Zdroj: | Cardiovascular Research. 47:759-768 |
| ISSN: | 0008-6363 |
| Popis: | Objective: Recent studies suggest that tranilast inhibits a variety of agents implicated in neointimal growth and restenosis in experimental animal models and humans. We report here a study evaluating the efficacy of tranilast in the rat carotid artery balloon angioplasty model, a model that mimics many aspects of the percutaneous transluminal angioplasty procedure in humans. Efficacy was determined based on in vivo and ex vivo magnetic resonance imaging (MRI) as well as by histomorphometry. The utility of this study, using a reverse paradigm, is to investigate if agents successful in the clinic can demonstrate efficacy in this animal model primary screen as measured by MRI and histomorphometry. Methods: Tranilast (300 mg/kg/day, p.o.) was administered to Sprague–Dawley rats 3 days prior to balloon injury and continued for 14 days after injury. Three methods of measuring the vascular injury that occurs in this model were employed: (1) in vivo MRI, used to measure in vivo lumen volumes for the carotid artery once at baseline (pre-surgery) and again at 14 days post angioplasty; (2) ex vivo MRI (and histomorphometry), used to evaluate the total arterial wall thickness and the intima-to-media ratio; and (3) analysis of collagen density, used to evaluate the efficacy of tranilast to abrogate collagen synthesis and deposition following vascular injury. Results: Tranilast provided 33% protection ( P |
| Databáze: | OpenAIRE |
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