Isolation, structure characteristics and antioxidant activity of two water-soluble polysaccharides from Lenzites betulina
| Autor: | Hongwei Dai, Yan Hua, Ling-Yun Zhou, Lei Guo, Jia-Yu Ma, Junmin Wang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Structure characteristics
DPPH Mannose Polysaccharide 01 natural sciences Deproteinization lcsh:Chemistry chemistry.chemical_compound Antioxidant activity Polysaccharides Monosaccharide Derivatization Purification chemistry.chemical_classification Chromatography biology 010405 organic chemistry Chemistry Lenzites betulina General Chemistry biology.organism_classification Glucuronic acid 0104 chemical sciences 010404 medicinal & biomolecular chemistry lcsh:QD1-999 Galactose Research Article |
| Zdroj: | BMC Chemistry, Vol 15, Iss 1, Pp 1-10 (2021) BMC Chemistry |
| Popis: | Background Fungal polysaccharides belong to a very important class of biological macromolecules in nature, and have complex monosaccharide composition and structure. These studies on structure and biological activity of fungal polysaccharides have become one of the research hotspots of scholars at home and abroad. Results This study was performed in order to understand the structural characteristics and antioxidant activity of polysaccharides from Lenzites betulina (LBPs). The LBPs were deproteinized using sevag method, and further purified by DEAE cellulose-52 column and Sephadex G-100 column chromatographies, then the two refined polysaccharides were obtained and named LBPs-5 and LBPs-6. Fourier transform infrared spectrometry (FT-IR) showed that LBPs-5 and LBPs-6 are typical β-pyranose with characteristic peaks of polysaccharides. The molecular weight of the two water-soluble polysaccharides were estimated to be 3.235 × 103 Da and 6.196 × 103 Da by HPGPC, respectively. HPLC with PMP derivatization analysis indicated that the monosaccharide compositions of LBPs-5 were mannose, glucuronic acid, glucose, and galactose in a molar ratio of 0.05:0.15:0.76:0.04. The monosaccharide compositions of LBPs-6 were mannose, glucuronic acid, and glucose, in a molar ratio of 0.04:0.17:0.79. Furthermore, the two water-soluble polysaccharides demonstrated strong scavenging effects on DPPH·, ABTS·+, ·OH and weak total reducing power, especially LBPs-6 was significantly stronger in scavenging rate than that of LBPs-5. Conclusions The outcome of the study indicated that LBPs had good potential as medicine and food. |
| Databáze: | OpenAIRE |
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