Mono-(2-ethylhexyl) phthalate (MEHP) affects intercellular junctions of Sertoli cell: A potential role of oxidative stress
| Autor: | Nogueira de Morais Rosana, Lustig Livia, Denduchis Berta, Cristian Marcelo Sobarzo, Helena Fedora Schteingart |
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| Rok vydání: | 2015 |
| Předmět: |
Male
Antioxidant CIENCIAS MÉDICAS Y DE LA SALUD medicine.medical_treatment Sertoli cells Ciencias de la Salud Nerve Tissue Proteins Biology Toxicology Occludin medicine.disease_cause Cell junction Antioxidants Lipid peroxidation Rats Sprague-Dawley chemistry.chemical_compound Diethylhexyl Phthalate Claudin-1 medicine oxidative stress Animals Cell Shape Cells Cultured MEHP Glutathione Transferase Sertoli Cells Tight junction Glutathione Sertoli cell Cadherins Cell biology Otras Ciencias de la Salud Oxidative Stress medicine.anatomical_structure Intercellular Junctions chemistry intercellular junctions Cytoprotection Connexin 43 Zonula Occludens-1 Protein Lipid Peroxidation Oxidative stress alpha Catenin |
| Zdroj: | Reproductive toxicology (Elmsford, N.Y.). 58 |
| ISSN: | 1873-1708 |
| Popis: | We analyzed the potential role of oxidative stress induced by mono (2-ethylhexyl) phthalate (MEHP) in adherent cell junction protein expression of prepubertal rat Sertoli cells (SC) in vitro. Five-day SC cultures were treated with MEHP (200 µM) for 24h and compared to cells in basal conditions. Western blot and immunofluorescent (IF) analyses showed that MEHP induced increase of N-cadherin and catenin expression, modifying its distribution. Concomitantly, Cx-43 expression decreased significantly and delocalization of the IF signal for tight junction proteins (occludin, claudin-11 and ZO-1) occurred. Indicative of oxidative stress, MEHP induced in SC an increase of lipoperoxides, a decrease in glutathione (GSH) levels and a concomitant increase in Glutathione S-Transferases (GST) activity. Antioxidant N-acetyl-cysteine (1mM) treatment prevented GSH decrease and N-cadherin and α-catenin up-regulation induced by MEHP. Our data suggest that oxidative stress signaling is a mechanism involved in adherent cell junctions disruption induced by MEHP in SC cultures. Fil: Sobarzo, Cristian M.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; Argentina Fil: Morais, Rosana Nogueira de. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; Argentina. Universidade Federal Do Parana; Brasil Fil: Lustig, Livia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; Argentina Fil: Denduchis, Berta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; Argentina Fil: Schteingart, Helena Fedora. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; Argentina |
| Databáze: | OpenAIRE |
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