Monitoring by HPLC of Chamomile Flavonoids Exposed to Rat Liver Microsomal Metabolism
| Autor: | Eva Szoke, Bernadett Benko, Ferenc Darvas, Huba Kalász, Georg Petroianu, P. Szegi, Kornélia Tekes, Rudolf Laufer |
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| Rok vydání: | 2009 |
| Předmět: |
Cytochrome
Flavonoid rutin Microsomal treatment Pharmaceutical Science High-performance liquid chromatography Article quercetin Rutin chemistry.chemical_compound Drug Discovery heterocyclic compounds Pharmacology chemistry.chemical_classification biology Metabolism apigenin-7-O-glucoside cytochrome P-450 dependent oxidation chemistry Biochemistry Lipophilicity Microsome biology.protein Molecular Medicine HPLC Quercetin |
| Zdroj: | The Open Medicinal Chemistry Journal |
| ISSN: | 1874-1045 |
| Popis: | Three major flavonoid chamomile components (quercetin, apigenin-7-O-glucoside and rutin) were subjected to oxidative metabolism by cytochrome P-450 of rat liver microsomal preparations. Changes over time in their respective concentrations were followed using reversed-phase HPLC with UV detection. No clean-up had to be applied as only the specific flavonoid had to be separated from the background components originating from the rat liver microsome. Neither the concentration of apigenin-7-O-glucoside nor that of the diglycoside rutin decreased during one hour of exposure to rat microsomal treatment. In contrast, the concentration of quercetin, a lipophilic aglycon, decreased. Our analytical HPLC results complement the in silico calculated lipophilicity (logP) of these compounds; the relatively high lipophilicity of quercetin appears to predispose it to oxidative metabolism in order to decrease its fat solubility. In contrast the much less lipophilic compounds apigenin-7-O-glucoside and rutin were resistant in vitro to microsomal treatment. |
| Databáze: | OpenAIRE |
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