Protective roles of thymoquinone and vildagliptin in manganese-induced nephrotoxicity in adult albino rats
| Autor: | Dalia Abdallah El-Shafei, Eman Ahmed Alaa El-Din, Aisha Abdallah Abouhashem, Nehal S. Abouhashem, Heba El-Sayed Mostafa |
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| Rok vydání: | 2020 |
| Předmět: |
Male
Health Toxicology and Mutagenesis 010501 environmental sciences Pharmacology medicine.disease_cause Kidney 01 natural sciences Antioxidants Nephrotoxicity Superoxide dismutase chemistry.chemical_compound medicine Benzoquinones Environmental Chemistry Animals Vildagliptin Thymoquinone 0105 earth and related environmental sciences Creatinine Manganese biology business.industry General Medicine Glutathione Pollution Rats Oxidative Stress medicine.anatomical_structure chemistry biology.protein business Oxidative stress medicine.drug |
| Zdroj: | Environmental science and pollution research international. 28(24) |
| ISSN: | 1614-7499 |
| Popis: | Despite being important in the body's mechanisms, excessive accumulation of manganese (Mn) can induce severe toxicity in vital organs of the body. Thymoquinone (TQ) is extracted from Nigella sativa seeds which recently gained popularity as dietary supplements and plant-based antioxidants. Vildagliptin (VLD) is a dipeptidyl peptidase IV (DPPIV) inhibitor, approved as anti-hyperglycemic agents with cardioprotective and renoprotective effects. The present study aimed to investigate the nephrotoxicity of Mn and the potential protective effects of thymoquinone and vildagliptin. Sixty-four adult male albino rats were equally divided into 8 groups: group I (control, received no medication), group II (vehicle, received normal saline), group III (TQ, 50 mg/kg/day), group IV (VLD, 10 mg/kg/day), group V (MnCl2, 50 mg/kg/day), group VI (Mn+TQ), group VII (Mn+VLD), and group VIII (Mn+TQ+VLD). Groups VI, VII, and VIII, received the same previously mentioned doses. All drugs were orally gavaged for 12 weeks. Manganese administration resulted in an elevation in the levels of serum and tissues Mn, blood glucose, serum urea, creatinine, tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and reduction in insulin, kidney superoxide dismutase (SOD), glutathione (GSH), and interleukin-10. Histopathological structural renal damage was detected associated with strong positive immunoexpression of caspase-3. On the other hand, individual or combined TQ and VLD administration with Mn significantly decreased the serum and tissue levels of Mn, declined the blood glucose, inflammatory markers, oxidative stress markers, ameliorated the histopathological effects, and down-regulated the immunoexpression of caspase-3. In conclusion, TQ and VLD co-administration elicited protective effects against Mn-induced nephrotoxicity. |
| Databáze: | OpenAIRE |
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