Experiments on the Nature of the Prothrombin-Converting Principle: Alteration of Proaccelerin Thrombin
| Autor: | Oscar D. Ratnoff, C. R. M. Prentice, Robert T. Breckenridge |
|---|---|
| Rok vydání: | 1967 |
| Předmět: |
Thrombin
Phospholipid Hirudin Factor V chemistry.chemical_element Hematology Calcium chemistry.chemical_compound Thrombin preparation chemistry Biochemistry Prothrombinase hemic and lymphatic diseases Factor X medicine Thromboplastin Prothrombin Blood Coagulation Phospholipids circulatory and respiratory physiology medicine.drug Proaccelerin |
| Zdroj: | British Journal of Haematology. 13:898-914 |
| ISSN: | 1365-2141 0007-1048 |
| DOI: | 10.1111/j.1365-2141.1967.tb08860.x |
| Popis: | SUMMARY An incubation mixture of activated Stuart factor, calcium, phospholipid and proaccelerin caused the generation of thrombin from prothrombin. The activity of this prothrombin- converting principle was measured by the rate at which it converted prothrombin to thrombin. In the absence of thrombin in the incubation mixture, a lag phase, following addition of the prothrombin-converting principle to prothrombin, was observed before thrombin formation occurred. But when a trace of thrombin was added to the incubation mixture, it converted the principle into an active form, causing the immediate formation of thrombin from prothrombin without any delay. It was demonstrated that thrombin had this action upon the prothrombin-converting principle by changing proaccelerin into a more active form. Thrombin-treated proaccelerin would not convert prothrombin to thrombin in the absence of activated Stuart factor, indicating that it is not, by itself, the prothrombin-converting principle. By purification of the thrombin preparation, and the use of hirudin, it was shown that the action of thrombin on proaccelerin was due to thrombin itself and not to activated Stuart factor or other contaminating substances in the thrombin preparation. |
| Databáze: | OpenAIRE |
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