Damage-Associated Molecular Patterns and Myeloid-Derived Suppressor Cells in Bronchoalveolar Lavage Fluid in Chronic Obstructive Pulmonary Disease Patients

Autor: Agata Nowicka, Tomasz Piorunek, Jan Sikora, Magdalena Wyrzykiewicz, Senan Yasar, Mariusz Kaczmarek, Beata Brajer-Luftmann, Halina Batura-Gabryel
Rok vydání: 2019
Předmět:
Male
lcsh:Immunologic diseases. Allergy
Article Subject
Immunology
Inflammation
Defensins
Pulmonary Disease
Chronic Obstructive
03 medical and health sciences
0302 clinical medicine
medicine
Humans
Immunology and Allergy
Hyaluronic Acid
Lung
Defensin
Heat-Shock Proteins
Aged
030304 developmental biology
0303 health sciences
COPD
Pulmonary Surfactant-Associated Protein A
medicine.diagnostic_test
business.industry
Myeloid-Derived Suppressor Cells
General Medicine
Middle Aged
respiratory system
medicine.disease
Acquired immune system
respiratory tract diseases
Bronchoalveolar lavage
medicine.anatomical_structure
030220 oncology & carcinogenesis
Myeloid-derived Suppressor Cell
Immunogenic cell death
Female
medicine.symptom
lcsh:RC581-607
business
Bronchoalveolar Lavage Fluid
Molecular Chaperones
Research Article
Zdroj: Journal of Immunology Research, Vol 2019 (2019)
Journal of Immunology Research
ISSN: 2314-7156
2314-8861
Popis: Myeloid-derived suppressor cells (MDSCs) are present in the human lung microenvironment, and they may be involved in the local inflammatory process in chronic obstructive pulmonary disease (COPD). Chronic inflammation in COPD may induce immunogenic cell death of structural airway cells, causing the release of damage-associated molecular patterns (DAMPs). DAMPs may activate the innate and adaptive immune system. The relationship between MDSCs and DAMPs in COPD is poorly described in the available literature.Objectives. (1) Assessment of MDSC percentage and DAMP concentration in bronchoalveolar lavage fluid (BALF) and peripheral blood. (2) Analysis of the relationship between MDSC percentage and chosen DAMPs.Patients and Methods. 30 COPD patients were included. Using monoclonal antibodies directly conjugated with fluorochromes in flow cytometry, MDSCs were assessed in BALF and peripheral blood. The concentration of DAMPs was estimated using sandwich ELISA. Using the Bradford method, the total protein concentrations were evaluated.Results. The percentage of MDSCs among MC in BALF correlated well with the concentration of defensin and heat shock protein 27. Assessing the percentage of MDSCs among all leukocytes in BALF, we revealed a significant correlation with the concentration of defensin, hyaluronic acid, and surfactant protein A. No dependencies occurred between DAMPs and MDSCs in peripheral blood.Conclusion. MDSCs and DAMPs occur in the COPD patient lung microenvironment. Significant correlations between them found in BALF may indicate their influence on the local inflammatory process in COPD. These relationships allow better understanding of the inflammatory process in COPD.
Databáze: OpenAIRE
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