Efficient transposition of Tn 4556 by alterations in inverted repeats using a delivery vector carrying a counter-selectable marker for Streptomyces
| Autor: | Akiko Sakoda, Masahiro Sota, Haruo Ikeda |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
DNA
Bacterial Genetics Transposable element Tn3 transposon Base Sequence Inverted repeat Sequence analysis Class-II transposon Transposases Bioengineering Sequence Analysis DNA Biology Applied Microbiology and Biotechnology Streptomyces Frameshift mutation Transposition (music) Open Reading Frames Genetics and Molecular Biology of Industrial Organisms - Short Communication Counter selection DNA Transposable Elements Phenylalanyl-t-RNA synthetase Selectable marker Transposase Plasmids Biotechnology |
| Zdroj: | Journal of Industrial Microbiology & Biotechnology |
| ISSN: | 1476-5535 1367-5435 |
| DOI: | 10.1007/s10295-018-2101-x |
| Popis: | A 6625-base pair transposon, Tn 4556, was initially isolated from a Streptomyces strain and a sequence analysis was performed; however, its annotation data remain incomplete. At least three positions were identified as frameshift and base-exchange errors by resequencing. The revised sequence revealed that Tn 4556 contains four open reading frames that encode transposase, methyltransferase, isoprenyl diphosphate transferase, and resolvase, respectively. Thirty-eight-base pair inverted repeat (IR) sequences at both ends contained a 1-bp mismatch flanked by a target duplication site, and transposition efficiency was improved by the replacement of imperfectly matched IR-L to perfectly matched IR-L. The detection of Tn 4556 transposition was markedly facilitated using a delivery vector carrying a strictly counter-selectable marker for Streptomyces strains. |
| Databáze: | OpenAIRE |
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