Vitamin B12 Absorption as Measured by Total B12 and Holo-TC Following A Single Oral Dose of Multivitamin in Healthy Adults
| Autor: | Kelly Zhang, Annahita Ghassemi, W Hooper, Laura Harkness |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
medicine.medical_specialty
Vitamins and Minerals Nutrition and Dietetics medicine.diagnostic_test business.industry Medicine (miscellaneous) Serum specimen Gastroenterology Intestinal absorption Single oral dose Single dose regimen Internal medicine Immunoassay Medicine Vitamin B12 absorption Vitamin B12 business Multivitamin Food Science |
| Zdroj: | Curr Dev Nutr |
| ISSN: | 2475-2991 |
| Popis: | OBJECTIVES: Measure total B12 and holotranscobalamin (Holo-TC) concentrations in serum to show vitamin B12 absorption following single oral doses of multivitamin (MVI) gummy and tablet. METHODS: This crossover clinical trial involved healthy adults randomized to either gummy or tablet multivitamins (MVI) containing vitamin B12 (60.8 mcg in gummy and 91.8 mcg in tablet) as cyanocobalamin in a single dose in Phase 1 with serial blood samples collected at pre-dose and at 0.5-, 1-, 2-, 4-, 6-, 8-, 9-, 10-, 24-, and 48-hrs after dosing, followed by a 2-week washout period. In Phase 2, participants then crossed over to receive MVI in the form not previously given, with blood draws at the same timepoints. Serum samples were measured for total B12 (n = 19) using an electrochemiluminescence immunoassay (ECLIA) and for Holo-TC (n = 14) using commercial Holo-TC ELISA kit. Measurements from groups of time points were averaged to estimate pre-absorptive (0 to 2 hours) and absorptive (6 to 10 hours) concentrations of total B12 and Holo-TC for each subject. The difference between pre-absorptive and absorptive concentrations for both analytes was calculated to estimate newly absorbed vitamin B12. RESULTS: Mean increases of about 15% in serum Holo-TC in absorptive phase over the pre-absorptive phase were observed for both the gummies and the tablets, which are statistically significant (p |
| Databáze: | OpenAIRE |
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