p75 Neurotrophin Receptor Activation Regulates the Timing of the Maturation of Cortical Parvalbumin Interneuron Connectivity and Promotes Juvenile-like Plasticity in Adult Visual Cortex
| Autor: | Laura Baroncelli, Elie Baho, Tommaso Pizzorusso, Raffaele Mazziotti, Fabrice Ango, Bidisha Chattopadhyaya, Celine Jahannault-Talignani, Marisol Lavertu-Jolin, Patricia N. Awad, Pegah N Chehrazi, Marianne Groleau, Elvire Vaucher, Graziella Di Cristo |
|---|---|
| Přispěvatelé: | Baho, E., Chattopadhyaya, B., Lavertu-Jolin, M., Mazziotti, R., Awad, P. N., Chehrazi, P., Groleau, M., Jahannault-Talignani, C., Vaucher, E., Ango, F., Pizzorusso, T., Baroncelli, L., Cristo, G. D. |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male Aging Critical period plasticity Cell Maturation Settore BIO/09 - Fisiologia Synapse Mice Random Allocation 0302 clinical medicine Ocular dominance plasticity Perineural net Sexual Maturation GABAergic Neurons P75NTR Research Articles Visual Cortex Neuronal Plasticity GABAergic perisomatic synapses Perineural nets PV cells General Neuroscience Gene Expression Regulation Developmental Recombinant Proteins Monocular deprivation medicine.anatomical_structure Parvalbumins Female Neurotrophin Interneuron GABAergic perisomatic synapse Receptors Nerve Growth Factor Biology Inhibitory postsynaptic potential 03 medical and health sciences Organ Culture Techniques Interneurons Vision Monocular Neuroplasticity medicine Connectome Animals Protein Precursors Brain-Derived Neurotrophic Factor Mice Inbred C57BL 030104 developmental biology Synapses biology.protein Evoked Potentials Visual sense organs Neuroscience 030217 neurology & neurosurgery Parvalbumin |
| Zdroj: | The Journal of neuroscience : the official journal of the Society for Neuroscience. 39(23) |
| ISSN: | 1529-2401 |
| Popis: | By virtue of their extensive axonal arborization and perisomatic synaptic targeting, cortical inhibitory parvalbumin (PV) cells strongly regulate principal cell output and plasticity and modulate experience-dependent refinement of cortical circuits during development. An interesting aspect of PV cell connectivity is its prolonged maturation time course, which is completed only by end of adolescence. The p75 neurotrophin receptor (p75NTR) regulates numerous cellular functions; however, its role on cortical circuit development and plasticity remains elusive, mainly because localizing p75NTR expression with cellular and temporal resolution has been challenging. By using RNAscope and a modified version of the proximity ligation assay, we found that p75NTR expression in PV cells decreases between the second and fourth postnatal week, at a time when PV cell synapse numbers increase dramatically. Conditional knockout of p75NTR in single PV neurons in vitro and in PV cell networks in vivo causes precocious formation of PV cell perisomatic innervation and perineural nets around PV cell somata, therefore suggesting that p75NTR expression modulates the timing of maturation of PV cell connectivity in the adolescent cortex. Remarkably, we found that PV cells still express p75NTR in adult mouse cortex of both sexes and that its activation is sufficient to destabilize PV cell connectivity and to restore cortical plasticity following monocular deprivation in vivo Together, our results show that p75NTR activation dynamically regulates PV cell connectivity, and represent a novel tool to foster brain plasticity in adults By virtue of their extensive axonal arborization and perisomatic synaptic targeting, cortical inhibitory parvalbumin (PV) cells strongly regulate principal cell output and plasticity and modulate experience-dependent refinement of cortical circuits during development. An interesting aspect of PV cell connectivity is its prolonged maturation time course, which is completed only by end of adolescence. The p75 neurotrophin receptor (p75NTR) regulates numerous cellular functions; however, its role on cortical circuit development and plasticity remains elusive, mainly because localizing p75NTR expression with cellular and temporal resolution has been challenging. By using RNAscope and a modified version of the proximity ligation assay, we found that p75NTR expression in PV cells decreases between the second and fourth postnatal week, at a time when PV cell synapse numbers increase dramatically. Conditional knockout of p75NTR in single PV neurons in vitro and in PV cell networks in vivo causes precocious formation of PV cell perisomatic innervation and perineural nets around PV cell somata, therefore suggesting that p75NTR expression modulates the timing of maturation of PV cell connectivity in the adolescent cortex. Remarkably, we found that PV cells still express p75NTR in adult mouse cortex of both sexes and that its activation is sufficient to destabilize PV cell connectivity and to restore cortical plasticity following monocular deprivation in vivo. Together, our results show that p75NTR activation dynamically regulates PV cell connectivity, and represent a novel tool to foster brain plasticity in adults. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|