Antioxidative Potential of Aqueous Extract of Ocimum americanum L. Leaves: An in vitro and in vivo Evaluation

Autor: B. T. Aluko, O. I. Oloyede
Jazyk: angličtina
Rok vydání: 2015
Předmět:
DOI: 10.5281/zenodo.1099392
Popis: Ocimum americanum L (Lamiaceae) is an annual herb that is native to tropical Africa. The in vitro and in vivo antioxidant activity of its aqueous extract was carefully investigated by assessing the DPPH radical scavenging activity, ABTS radical scavenging activity and hydrogen peroxide radical scavenging activity. The reducing power, total phenol, total flavonoids and flavonols content of the extract were also evaluated. The data obtained revealed that the extract is rich in polyphenolic compounds and scavenged the radicals in a concentration dependent manner. This was done in comparison with the standard antioxidants such as BHT and Vitamin C. Also, the induction of oxidative damage with paracetamol (2000 mg/kg) resulted in the elevation of lipid peroxides and significant (P < 0.05) decrease in activities of superoxide dismutase, glutathione peroxidase, glutathione reductase and catalase in the liver and kidney of rats. However, the pretreatment of rats with aqueous extract of O. americanum leaves (200 and 400 mg/kg) and silymarin (100 mg/kg) caused a significant (P < 0.05) reduction in the values of lipid peroxides and restored the levels of antioxidant parameters in these organs. These findings suggest that the leaves of O. americanum have potent antioxidant properties which may be responsible for its acclaimed folkloric uses.
{"references":["A. Jayachitra and N. Krithiga, \"Study on antioxidant property in selected\nmedicinal plant extract,\" International Journal of Medicinal and\nAromatic Plants, vol. 2, no. 3, pp. 495–500, 2010.","K. Hensley, K.A. Robinson, S.P. Gabbita, S. Salsman, and R.A. Floyd,\n''Reactive oxygen species, cell signaling, and cell injury'', Free Radic\nBiol Med. Vol. 28, no. 10, pp. 1456–1462, 2000.","A. Manea, A. Fortuno and J. L. Martin-Ventura, \"Oxidative stress in\ncardiovascular pathologies: genetics, cellular, and molecular\nmechanisms and future antioxidant therapies'', Oxid. Med. Cell Longev.\nvol. 2012, pp. 373450, 2012.","J. F. Turrens, \"Mitochondrial formation of reactive oxygen species\", J.\nPhysiol. Vol. 552, no. 2, pp. 335–344, 2003.","V. R. Patel, P. R. Patel, and S. S. Kajal, \"Antioxidant activity of some\nselected medicinal plants in western region India,\" Advances in\nBiological Research, vol. 4, pp. 23–26, 2010.","F. Aqil, I. Ahmad, and Z. Mehmood, \"Antioxidant and free radical\nscavenging properties of twelve traditionally used Indian medicinal\nplants,\" Turkish Journal of Biology, vol. 30, no. 3, pp. 177–183, 2006.","S. A. Ganie, A. Haq, A. Masood, A. Hamid, and M. A. Zargar,\n\"Antioxidant and protective effect of ethyl acetate extract of\nPodophyllum hexandrum rhizome on carbon tetrachloride induced rat\nliver injury\", Evid. Based. Complement. Altern. Med. Vol. 2011 pp. 1 –\n12, 2011","J. Steel, \"Perfumeros and the sacred use of fragrance in amazonian\nshamanism\". The smell culture reader, edited by Jim Drobnick Berg\npublishers.2006; Pp 23.","N.J. Hogarh, \"Effect of Ocimum canum aqueous extract on experimental\ndiabetes mellitus. BSc Research Project Report, Department of\nBiochemistry, University of Ghana, Accra.1996.\n[10] I.H.N. Bassole, R. Nebie, A. Savadogo, C.T. Ouattara, N. Barro and\nS.A. Traore, \"Composition and antimicrobial activities of the leaf and\nflower essential oils of Lippia chevalieri and Ocimum canum from\nBukinafaso\", Afr. J. Biotech. 2005, vol. 4 no. 10, pp. 1156-1160, 2005.\n[11] G. Oboh, \"Antioxidative potential of Ocimum gratissimum and Ocimum\ncanum leaf polyphenols and protective effects on some pro-oxidants\ninduced lipid peroxidation in rat brain: An in vitro study\", American J.\nFood Technol. vol.3 no. 5, pp. 325-334, 2008.\n[12] B.T. Aluko, O.I. Oloyede and A.J. Afolayan , \"Polyphenolic contents\nand free radical scavenging potential of extracts from leaves of Ocimum\namericanum L\", Paki. J. Biol. Sci. vol. 16 no. 1, pp. 22 – 30, 2013\n[13] A..A..L. Ordonez, J.D. Gomez, M.A. Vattuone and M.I. Isla,\n\"Antioxidant activities of Sechiumedule (Jacq.) Swartz extracts\", Food\nChem. Vol. 97, pp. 452-458, 2006.\n[14] A. Kumaran and R.J. Karunakaran, \"In vitro antioxidant activities of\nmethanol extracts of five Phyllanthus species from India\", Food Sci.\nTechnol. vol. 40, pp. 344-352, 2007.\n[15] Q.A. Nazari, T. Kume, N. Izuo, Y. Takada-Takatori, A. Imaizumi, T.\nHashimoto, Y. Izumi, A. Akaike, \"Neuroprotective effects of curcumin\nand highly bioavailable curcumin on oxidative stress induced by sodium\nnitroprusside in rat striatal cell culture\", Biol. Pharm. Bull. Vol. 36, no.8,\npp. 1356–1362, 2013.\n[16] R. Re, N. Pellegrini, A. Proteggente, A. Pannala, M. Yang and C. Rice-\nEvans, \"Antioxidant activity applying an improved ABTS radical cation\ndecolorization assay\" Free Radic. Biol. Med. Vol. 26, pp. 1231-1237,\n1999.\n[17] S. J. Heo, E.J. Park, K.W. Lee and Y.J. Jeon, \"Antioxidant activities of\nenzymatic extracts from brown seaweeds\", Bioresour. Technol. vol. 96,\nno. 14, pp. 1613–1623, 2005.\n[18] W.G. Niehaus and B. Samuelsson, \"Formation of malondialdehyde from\nphospholipids arachidonate during microsomal lipid peroxidation\" Eur.\nJ. Biochem. Vol. 6, pp. 126-130, 1968\n[19] D.E. Paglia and W.N. Valentine, \"Studies on the quantitative and\nqualitative characterization of erythrocyte glutathione peroxidase\" Lab.\nClin. Med. Vol. 70, pp. 158-169, 1967.\n[20] D.M. Goldberg and R.J. Spooner, \"Glutathione reductase.. In:\nBergmeyer HU, Bergmeyer J GraBl M.(ed). Methods of enzymatic\nanalysis, 3rd ed., vol. 111. Verlag Chemie, Weinheim, pp 258 -265, 1983.\n[21] A.K. Sinha, \"Colorimetric assay of catalase\", Anal. Biochem. Vol. 47,\npp. 389 – 394, 1972.\n[22] J. Iqbal, S. Zaib, U. Farooq, A. Khan and I. Bibi, \"Antioxidant,\nAntimicrobial, and Free Radical Scavenging Potential of Aerial Parts of\nPeriploca aphylla and Ricinus communis\", ISRN Pharmacol. Vol. 2012,\npp. 563267, 2012.\n[23] G. Milliauskas, P.R. Venskutonis and T.A. Van-Beek, \"Screening of\nradical scavenging activity of some medicinal and aromatic plant\nextracts\" Food Chem. Vol. 85, pp. 231-237, 2004.\n[24] S.O. Oyedemi, G. Bradley and A.J. Afolayan, \"In vitro and in vivo\nantioxidant activities of aqueous extract of Strychnos henningsii Gilg\",\nAfr. J. Pharm. Pharmacol. Vol. 4, pp. 70-78, 2010.\n[25] R.B. Walker and J.O. Everette, \"Comparative reaction rates of various\nantioxidants with ABTS radical cation\" J. Agric. Food Chem. Vol. 57,\nno. 4, 1156-1161, 2009.\n[26] K.H. Reddy, P.V.G.K Aharma and O.V.S. Reddy, \"A comparative in\nvitro study on antifungal and antioxidant activities of Nervilia aragoana\nand Atlantia monophylla\", Pharm. Biol. Vol. 48, pp. 595-602, 2010.\n[27] S.G. Hart, W.P. Beierschmitt, D.S. Wyand, E.A. Khairallah, S.D. Cohen,\n\"Acetaminophen nephrotoxicity in CD-1 mice. I. Evidence of a role for\nin situ activation in selective covalent binding and toxicity\" Toxicol.\nAppl. Pharmacol. Vol. 126, no. 2, 267-275, 1994.\n[28] K. Boutis and M. Shannon, \"Nephrotoxicity after acute severe\nacetaminophen poisoning in adolescents\" J. Toxicol. Clin. Toxicol. Vol.\n39, no. 5, pp. 441-445, 2001.\n[29] Z. Abdul Hamid, B. B. Siti, J. Ng Wen, H. Asmah, H. Khairana, and\nJamaludin, M. \"Nephroprotective effects of Zingiber zerumbet Smith\nethyl acetate extract against paracetamol-induced nephrotoxicity and\noxidative stress in rats\" J. Zhejiang Univ-Sci. B. (Biomed. &\nBiotechnol.) vol. 13, no. 3, 176-185, 2012.\n[30] M. Kanter, I. Meral, S. Dede, H. Gunduz, M. Cemek, H. Ozbek and I.\nUygan, \"Effects of Nigella sativa L. and Urtica dioica L. on lipid\nperoxidation, antioxidant enzyme systems and some liver enzymes in\nCCl4-treated rats\", J. Vet. Med. Physiol. Pathol. Clin. Med. Vol. 50, pp.\n264-268, 2003.\n[31] S. Santhosh, R Anandan, T.K. Sini, P.T. Mathew and T.K. Thankappan,\n\"Biochemical studies on the antiulcer effect of glucosamine on\nantioxidant defense status in experimentally induced peptic ulcer in\nrats\", J. Glin. Biochem. Nutr. Vol.37, pp. 61 – 66, 2005"]}
Databáze: OpenAIRE
Externí odkaz: