Validation of a new yeast-based reporter assay consisting of human estrogen receptors α/β and coactivator SRC-1: Application for detection of estrogenic activity in environmental samples
| Autor: | Mami Kondo, Wai-Ling Chu, Takashi Yagi, Masanobu Kawanishi, Kazuhiro Shiizaki |
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| Rok vydání: | 2009 |
| Předmět: |
endocrine system
Transcription Genetic medicine.drug_class Health Toxicology and Mutagenesis Estrogen receptor Saccharomyces cerevisiae Management Monitoring Policy and Law Biology Ligands Toxicology chemistry.chemical_compound Nuclear Receptor Coactivator 1 Genes Reporter Coactivator medicine Estrogen Receptor beta Receptor Histone Acetyltransferases Reporter gene Dose-Response Relationship Drug Estrogen Receptor alpha Estrogens General Medicine Nuclear receptor coactivator 1 chemistry Biochemistry Endocrine disruptor Estrogen Biological Assay Phytoestrogens Water Pollutants Chemical hormones hormone substitutes and hormone antagonists Environmental Monitoring Transcription Factors |
| Zdroj: | Environmental Toxicology. 24:513-521 |
| ISSN: | 1522-7278 1520-4081 |
| Popis: | Endocrine disruptors are exogenous substances that act like hormones in the endocrine system and disrupt the physiologic function of endogenous hormones. In the present study, we established reporter yeast strains (Saccharomyces cerevisiae) expressing human estrogen receptors, ERalpha or ERbeta. These strains contain a reporter plasmid carrying an estrogen responsive element (ERE) upstream of the beta-galactosidase gene, and a plasmid expressing a steroid receptor coactivator, SRC-1e. Using these reporter strains, we demonstrated dose-dependent estrogenic activities of different categories of ligands, a natural hormone, 17beta-estradiol (E2); a synthetic drug, diethylstilbestrol (DES); phytoestrogens, genistein, daizein and emodin; and an environmental endocrine disrupter, bisphenol A. EC(50) values of E2 for ERalpha and ERbeta are 5.31 x 10(-10) and 5.85 x 10(-10) M, respectively. We also demonstrated that these yeasts were applicable for measuring estrogenic activities of environmental water samples. Most downstream sites of a river showed similar activity in both ERalpha and ERbeta assays. These yeast strains are useful and convenient for detecting and comparing the estrogenic ligand activities of environmental samples in response to ERalpha and ERbeta. |
| Databáze: | OpenAIRE |
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