O-GlcNAcylation of BMAL1 regulates circadian rhythms in NIH3T3 fibroblasts
| Autor: | Hunjin Luo, Wen-Juan Guan, Jia-Da Li, Yan-Tao Ma, Ziyao Wang, Chongfen Chen, Hua Zhang |
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| Rok vydání: | 2012 |
| Předmět: |
Transcription
Genetic Acylation Circadian clock Biophysics Biology Biochemistry Acetylglucosamine Mice Transcription (biology) Genes Reporter Animals Humans Circadian rhythm Luciferases Molecular Biology Protein Stability HEK 293 cells ARNTL Transcription Factors Cell Biology Metabolism Fibroblasts Bacterial circadian rhythms Cell biology Circadian Rhythm CLOCK HEK293 Cells Light effects on circadian rhythm NIH 3T3 Cells |
| Zdroj: | Biochemical and biophysical research communications. 431(3) |
| ISSN: | 1090-2104 |
| Popis: | Various physiological processes and behaviors show a circadian rhythm of approximately 24 h, which is crucial in coordinating internal metabolic processes and environmental signals. Post-translational modifications play an important role in regulating circadian core proteins. In this study, we demonstrated that BMAL1 was modified with an O-linked β-N-acetylglucosamine (O-GlcNAc), which stabilized BMAL1 and enhanced its transcriptional activity. Conversely, inhibition of O-GlcNAcylation resulted in inhibition of circadian rhythms of clock gene expression. Because O-GlcNAcylation is sensitive to the glucose level, such a modification may provide a new mechanism connecting metabolism with circadian rhythms. |
| Databáze: | OpenAIRE |
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