The ric-8a/galpha13/fak signaling cascade controls focal adhesion formation during neural crest cell migration
| Autor: | Roberto Mayor, Andréa Beyer, Sylvain Marcellini, Alice Roycroft, Marcela Torrejón, Gabriela Toro-Tapia, Soraya Villaseca |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Embryo Nonmammalian Xenopus Down-Regulation Xenopus Proteins Biology GTP-Binding Protein alpha Subunits G12-G13 Models Biological Morpholinos Focal adhesion 03 medical and health sciences 0302 clinical medicine Cranial neural crest Cell Movement hemic and lymphatic diseases Cell Adhesion Animals Guanine Nucleotide Exchange Factors Cell adhesion Molecular Biology Focal Adhesions Cell Membrane Neural crest Morphant Cell migration Actin cytoskeleton Cell biology Actin Cytoskeleton Phenotype src-Family Kinases 030104 developmental biology Neural Crest Focal Adhesion Protein-Tyrosine Kinases Neural crest cell migration 030217 neurology & neurosurgery Research Article Signal Transduction Developmental Biology |
| Zdroj: | Develpment Artículos CONICYT CONICYT Chile instacron:CONICYT |
| Popis: | Ric-8A is a pleiotropic guanine nucleotide exchange factor involved the activation of various heterotrimeric G protein pathways during adulthood and early development. Here, we sought to determine the downstream effectors of Ric-8A during the migration of the vertebrate cranial neural crest (NC) cells. We show that the Gα13 knockdown phenocopies the Ric-8A morphant condition, causing actin cytoskeleton alteration, protrusion instability and a strong reduction in the number and dynamics of focal adhesions. In addition, the overexpression of Gα13 is sufficient to rescue Ric-8A depleted cells. Ric-8A and Gα13 physically interact and co-localize in protrusions of the cells leading edge. The focal adhesion kinase FAK co-localizes and interacts with the endogenous Gα13, and a constitutively active form of Src efficiently rescues the Gα13 morphant phenotype in NC cells. We propose that Ric-8A-mediated Gα13 signaling is required for proper cranial NC cell migration by regulating focal adhesion dynamics and protrusion formation. |
| Databáze: | OpenAIRE |
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