Rho-kinase levels in testicular ischemia-reperfusion injury and effects of its inhibitor, Y-27632, on oxidative stress, spermatogenesis, and apoptosis
| Autor: | Nil Doğruer Ünal, Erdem Akbay, Selahittin Çayan, Nalan Tiftik, Kansu Büyükafşar, Barış Saylam, Duygu Düşmez Apa, Ozan Efesoy, Burak Çimen, Murat Bozlu |
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| Rok vydání: | 2013 |
| Předmět: |
Male
medicine.medical_specialty Pyridines Urology Ischemia Apoptosis medicine.disease_cause Antioxidants chemistry.chemical_compound Internal medicine Testis medicine Animals Humans Enzyme Inhibitors Rats Wistar Spermatogenesis Rho-associated protein kinase Spermatic Cord Torsion rho-Associated Kinases business.industry medicine.disease Amides Rats Y-27632 Oxidative Stress Endocrinology Terminal deoxynucleotidyl transferase chemistry Reperfusion Injury business Reperfusion injury Oxidative stress |
| Zdroj: | Urology. 83(3) |
| ISSN: | 1527-9995 |
| Popis: | Objective To investigate testicular Rho-kinase levels and the effects of its inhibitor, Y-27632, on oxidative stress, spermatogenesis, and apoptosis in testicular ischemia-reperfusion rat model. Methods The study included 29 adult Wistar-Albino male rats weighing 150-200 g. The rats were divided into 3 groups. Group 1 underwent sham operation (n = 10). In group 2, left testicular torsion-detorsion was performed (n = 9). In group 3, Rho-kinase inhibitor Y-27632 (5 mg/kg) was injected intraperitoneally 30 minutes before detorsion (n = 10). Two months later, bilateral orchiectomy was performed in all the groups. Rho-kinase levels by Western blotting, apoptosis with terminal deoxynucleotidyl transferase dUTP nick end labeling method, testicular damage and spermatogenesis with modified Johnsen score, testicular total antioxidative status, and total oxidative status were measured. Results In the torsion-detorsion (T/D) group, Rho-kinase level increased significantly, compared with the sham group ( P = .025). In the Y-27632 treatment group, Johnsen scores were significantly higher, and apoptosis indexes were significantly lower, compared with the T/D group ( P = .001). Significantly higher total antioxidative status levels and lower total oxidative status levels were observed in the Y-27632 treatment group, compared with the T/D group ( P = .001 and P = .002, respectively). Conclusion Testicular ischemia-reperfusion significantly increased Rho-kinase levels in rats, and administration of Rho-kinase inhibitor, Y-27632, before detorsion might prevent ischemia-reperfusion injury. |
| Databáze: | OpenAIRE |
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