Evaluation of the Prevalence and Production of Escherichia coli Common Pilus among Avian Pathogenic E. coli and Its Role in Virulence

Autor: Melha Mellata, Roy Curtiss rd, Laura Durán, Miguel A. De la Cruz, Natalie M. Mitchell, Jorge A. Girón, Jacob T. Maddux, Alyssa K. Stacy
Rok vydání: 2014
Předmět:
Operon
Veterinary Microbiology
lcsh:Medicine
medicine.disease_cause
Polymerase Chain Reaction
Pilus
law.invention
fluids and secretions
Cell Movement
Pathogenic Escherichia coli
law
Prevalence
lcsh:Science
Escherichia coli Infections
Polymerase chain reaction
2. Zero hunger
Multidisciplinary
Virulence
Zoonotic Diseases
Escherichia coli Proteins
Animal Models
Veterinary Bacteriology
Chicken
Bacterial Pathogens
Phenotype
Infectious Diseases
Veterinary Diseases
Medicine
Research Article
DNA
Bacterial
Veterinary Medicine
animal structures
Virulence Factors
Blotting
Western
education
Biology
Microbiology
Model Organisms
Escherichia coli
Genetics
medicine
Animals
Humans
Microbial Pathogens
Gene
Poultry Diseases
lcsh:R
Biofilm
biology.organism_classification
Virology
Fimbriae
Bacterial
Veterinary Science
lcsh:Q
Gene Function
Chickens
HeLa Cells
Zdroj: PLoS ONE, Vol 9, Iss 1, p e86565 (2014)
PLoS ONE
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0086565
Popis: Avian pathogenic Escherichia coli (APEC) strains cause systemic and localized infections in poultry, jointly termed colibacillosis. Avian colibacillosis is responsible for significant economic losses to the poultry industry due to disease treatment, decrease in growth rate and egg production, and mortality. APEC are also considered a potential zoonotic risk for humans. Fully elucidating the virulence and zoonotic potential of APEC is key for designing successful strategies against their infections and their transmission. Herein, we investigated the prevalence of a newly discovered E. coli common pilus (ECP) for the subunit protein of the ECP pilus (ecpA) and ECP expression amongst APEC strains as well as the role of ECP in virulence. A PCR-based ecpA survey of a collection of 167 APEC strains has shown that 76% (127/167) were ecpA+. An immunofluorescence assay using anti-EcpA antibodies, revealed that among the ecpA+ strains, 37.8% (48/127) expressed ECP when grown in DMEM +0.5% Mannose in contact with HeLa cells at 37°C and/or in biofilm at 28°C; 35.4% (17/48) expressed ECP in both conditions and 64.6% (31/48) expressed ECP in biofilm only. We determined that the ecp operon in the APEC strain χ7122 (ecpA+, ECP-) was not truncated; the failure to detect ECP in some strains possessing non-truncated ecp genes might be attributed to differential regulatory mechanisms between strains that respond to specific environmental signals. To evaluate the role of ECP in the virulence of APEC, we generated ecpA and/or ecpD-deficient mutants from the strain χ7503 (ecpA+, ECP+). Deletion of ecpA and/or ecpD abolished ECP synthesis and expression, and reduced biofilm formation and motility in vitro and virulence in vivo. All together our data show that ecpA is highly prevalent among APEC isolates and its expression could be differentially regulated in these strains, and that ECP plays a role in the virulence of APEC.
Databáze: OpenAIRE
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