A complex consisting of pp60c-src/pp60c-srcN and a 38 kDa protein is highly enriched in growth cones from differentiated SH-SY5Y neuroblastoma cells
| Autor: | G. Meyerson, K.H. Pfenninger, S. Pahlman |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
animal structures
Cellular differentiation Proto-Oncogene Proteins pp60(c-src) Cell Biology Cell Fractionation Proto-Oncogene Mas Gene product Neuroblastoma Tumor Cells Cultured medicine Humans Growth cone Neurons Gel electrophoresis Genetics Cell Differentiation Cell Biology Phosphoproteins In vitro Cell biology Molecular Weight Microscopy Electron medicine.anatomical_structure Cell culture Tetradecanoylphorbol Acetate Neuron |
| Zdroj: | Journal of Cell Science. 103:233-243 |
| ISSN: | 1477-9137 0021-9533 |
| DOI: | 10.1242/jcs.103.1.233 |
| Popis: | Nerve growth cones of primary neurons are highly enriched in the proto-oncogene product pp60c-src. In order to investigate this molecule further in growing neuronal cells, growth cone and cell body fractions were prepared from human SH-SY5Y neuroblastoma cells differentiated neuronally in vitro under the influence of phorbol ester. The fractions were characterized ultrastructurally and by biochemical criteria. The neuronal (pp60c-srcN) and the fibroblastic (pp60c-src) forms of pp60src are slightly enriched and activated in the growth cones relative to the perikarya. Immunoprecipitates of pp60src from differentiated SH-SY5Y growth cones contain at least four phosphoproteins in addition to pp60src. One of these, pp38, migrates as a 100–140 kDa complex with pp60src under non-reducing conditions of gel electrophoresis. The pp38/pp60src complex is not easily detected in non-differentiated SH-SY5Y cells or perikarya of differentiated SH-SY5Y cells, but it is highly enriched in the growth cone preparation. These data suggest that growth-cone pp60src exists in a disulfide-linked oligomeric complex. The complex appears to be assembled only in the cell periphery and may be dependent upon neuronal differentiation. |
| Databáze: | OpenAIRE |
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