Equilibrium Sedimentation of Turnip Yellow Mosaic Virus
Autor: | W. Godschalk, D. W. Kupke, Jesse W. Beams, J. H. McGee |
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Rok vydání: | 1974 |
Předmět: |
Analytical chemistry
Plant Viruses law.invention Magnetics Viral Proteins Excluded volume effect Mosaic Viruses law Multidisciplinary Turnip yellow mosaic virus Mosaic virus biology Chemistry Rotor (electric) Hydrogen-Ion Concentration Sedimentation biology.organism_classification Molecular Weight Crystallography Sedimentation equilibrium RNA Viral Particle Biological Sciences: Biochemistry Ultracentrifuge Ultracentrifugation Densitometry |
Zdroj: | Proceedings of the National Academy of Sciences. 71:3866-3868 |
ISSN: | 1091-6490 0027-8424 |
DOI: | 10.1073/pnas.71.10.3866 |
Popis: | Sedimentation equilibrium was achieved with turnip yellow mosaic virus at low speeds (600 rpm) in a magnetic ultracentrifuge. The experiments were carried out in the newly installed constant-speed rotor, equipped with automatic control and an electromagnetic drive. A particle mass of 5.55 × 10 6 daltons was calculated for the virus at vanishing concentrations, in essential agreement with the earlier results using the more tedious procedure with a freely coasting rotor. In order to interpret the observed departure from ideal behavior (nonassociating conditions), preferential interaction experiments were carried out by magnetic densimetry. These showed that a strong Donnan effect exists at pH 7, which contributed > 10 3 times more to the nonideality than did the excluded volume effect. A net negative charge of about 3.5 × 10 3 per particle was estimated at this pH. |
Databáze: | OpenAIRE |
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