Prevalence of pathogenic free-living amoeba and other protozoa in natural and communal piped tap water from Queen Elizabeth protected area, Uganda
| Autor: | Kevin Muwonge, Joseph Erume, Charles Drago Kato, Celsus Sente, Sylvester Ochwo, Julius Mulindwa, George Sebyatika, Benigna Gabriela Namara, Michael Ocaido, Phillip Kimuda Magambo, Irene Naigaga |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Giardiasis 030106 microbiology Cryptosporidiosis Cryptosporidium Naegleria Microbiology 03 medical and health sciences Protozoan infection parasitic diseases medicine Prevalence RNA Ribosomal 18S Humans Uganda Protozoa biology Amoebida Drinking Water Giardia Public Health Environmental and Occupational Health Water Queen Elizabeth General Medicine Amebiasis Sequence Analysis DNA DNA Protozoan biology.organism_classification medicine.disease Free-living Acanthamoeba 030104 developmental biology Infectious Diseases Hartmannella GIARDIA SPP Research Article |
| Zdroj: | Infectious Diseases of Poverty |
| ISSN: | 2049-9957 |
| Popis: | Background Pathogenic water dwelling protozoa such as Acanthamoeba spp., Hartmannella spp., Naegleria spp., Cryptosporidium spp. and Giardia spp. are often responsible for devastating illnesses especially in children and immunocompromised individuals, yet their presence and prevalence in certain environment in sub-Saharan Africa is still unknown to most researchers, public health officials and medical practitioners. The objective of this study was to establish the presence and prevalence of pathogenic free-living amoeba (FLA), Cryptosporidium and Giardia in Queen Elizabeth Protected Area (QEPA). Methods Samples were collected from communal taps and natural water sites in QEPA. Physical water parameters were measured in situ. The samples were processed to detect the presence of FLA trophozoites by xenic cultivation, Cryptosporidium oocysts by Ziehl-Neelsen stain and Giardia cysts by Zinc Sulphate floatation technique. Parasites were observed microscopically, identified, counted and recorded. For FLA, genomic DNA was extracted for amplification and sequencing. Results Both natural and tap water sources were contaminated with FLA, Cryptosporidium spp. and Giardia spp. All protozoan parasites were more abundant in the colder rainy season except for Harmannella spp. and Naegleria spp. which occurred more in the warmer months. The prevalence of all parasites was higher in tap water than in natural water samples. There was a strong negative correlation between the presence of Acanthamoeba spp., Hartmannella spp., Cryptosporidium spp. and Giardia spp. with Dissolved Oxygen (DO) (P |
| Databáze: | OpenAIRE |
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