Dynamic Imaging Reveals Coordinate Effects of Cyclic Stretch and Substrate Stiffness on Endothelial Integrity
| Autor: | Deborah E. Leckband, Ryan B. Huang, Arkaprava Dan |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Fluorescence-lifetime imaging microscopy Materials science Dynamic imaging Biomedical Engineering Nanotechnology 02 engineering and technology Mechanotransduction Cellular Article 03 medical and health sciences Monolayer Substrate stiffness medicine Humans Mechanotransduction Actin Cells Cultured Fluorescent Dyes Thrombin Stiffness Endothelial Cells 021001 nanoscience & nanotechnology 030104 developmental biology Membrane Biophysics Endothelium Vascular Stress Mechanical medicine.symptom 0210 nano-technology |
| Popis: | We describe an equibiaxial cell stretcher and hybrid, elastic membrane platform designed for dynamic imaging of cells on substrates with physiological stiffness undergoing cyclic stretch. Studies enabled by this device revealed that both substrate stiffness and cyclic stretch coordinately protect pulmonary endothelial monolayers against thrombin-induced disruption. The fluorescence imaging possible with the designed hybrid membranes further revealed similarities and differences in actin and cell dynamics during monolayer recovery. The improved live-cell imaging capabilities of this platform, when used in conjunction with fluorescent probes, will have broad applications for investigations of the impact of biochemical stimuli and mechanotransduction mechanisms on mechanically perturbed tissues. |
| Databáze: | OpenAIRE |
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