Isolation and Establishment of a Highly Proliferative, Cancer Stem Cell-Like, and Naturally Immortalized Triple-Negative Breast Cancer Cell Line, KAIMRC2
| Autor: | Mohamed Boudjelal, Musaad A. Alshammari, Hajar Al Zahrani, Lolwah Al Riyees, Mohammed Al Balwi, Hassan Al Nikhli, Tlili Barhoumi, Rizwan Ali, Alshaimaa Alhallaj, Abdul Latif Khan, Yasser A. Alshawakir, Omar Baz, Abdullah H. Alanazi, Abdullah Mashhour |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Adult
Proliferative index QH301-705.5 Triple Negative Breast Neoplasms Article triple negative Breast cancer breast cancer Cancer stem cell stem cells Cell Line Tumor medicine Biomarkers Tumor Humans characterization Biology (General) Triple-negative breast cancer Cell Proliferation biology CD44 KAIMRC2 Cancer drug treatment General Medicine cell line medicine.disease Neoplasm Proteins metastatic Cell culture Cancer research biology.protein Neoplastic Stem Cells Female Stem cell |
| Zdroj: | Cells Volume 10 Issue 6 Cells, Vol 10, Iss 1303, p 1303 (2021) |
| ISSN: | 2073-4409 |
| DOI: | 10.3390/cells10061303 |
| Popis: | In vitro studies of a disease are key to any in vivo investigation in understanding the disease and developing new therapy regimens. Immortalized cancer cell lines are the best and easiest model for studying cancer in vitro. Here, we report the establishment of a naturally immortalized highly tumorigenic and triple-negative breast cancer cell line, KAIMRC2. This cell line is derived from a Saudi Arabian female breast cancer patient with invasive ductal carcinoma. Immunocytochemistry showed a significant ratio of the KAIMRC2 cells’ expressing key breast epithelial and cancer stem cells (CSCs) markers, including CD47, CD133, CD49f, CD44, and ALDH-1A1. Gene and protein expression analysis showed overexpression of ABC transporter and AKT-PI3Kinase as well as JAK/STAT signaling pathways. In contrast, the absence of the tumor suppressor genes p53 and p73 may explain their high proliferative index. The mice model also confirmed the tumorigenic potential of the KAIMRC2 cell line, and drug tolerance studies revealed few very potent candidates. Our results confirmed an aggressive phenotype with metastatic potential and cancer stem cell-like characteristics of the KAIMR2 cell line. Furthermore, we have also presented potent small molecule inhibitors, especially Ryuvidine, that can be further developed, alone or in synergy with other potent inhibitors, to target multiple cancer-related pathways. |
| Databáze: | OpenAIRE |
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