Smoking and fluidity of erythrocyte membranes: A high resolution scanning electron and atomic force microscopy investigation
| Autor: | Jeanette N. du Plooy, Antoinette V. Buys, Ina Keyser, Prashilla Soma, Etheresia Pretorius |
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| Rok vydání: | 2013 |
| Předmět: |
Adult
Male Cancer Research Erythrocytes Adolescent Membrane Fluidity Physiology Scanning electron microscope Clinical Biochemistry Phospholipid Nanotechnology Microscopy Atomic Force Biochemistry law.invention Young Adult chemistry.chemical_compound Superoxides law Electron microscopy Humans Aged Membranes Chemistry Atomic force microscopy Erythrocyte Membrane Smoking Resolution (electron density) Middle Aged Rbc membrane Membrane Microscopy Electron Scanning Biophysics Female General health Electron microscope |
| Zdroj: | Nitric Oxide. 35:42-46 |
| ISSN: | 1089-8603 |
| DOI: | 10.1016/j.niox.2013.08.003 |
| Popis: | Smoking affects the general health of an individual, however, the red blood cells (RBCs) and their architecture are particularly vulnerable to inhaled toxins related to smoking. Smoking is one of the lifestyle diseases that are responsible for the most deaths worldwide and an individual who smokes is exposed to excessive amounts of oxidants and toxins which generate up to 1018 free radicals in the human body. Recently, it was reported that smoking decreases RBC membrane fluidity. Here we confirm this and we show changes visible in the topography of RBC membranes, using scanning electron microscopy (SEM). RBC membranes show bubble formation of the phospholipid layer, as well as balloon-like smooth areas; while their general discoid shapes are changed to form pointed extensions. We also investigate membrane roughness using atomic force microscopy (AFM) and these results confirm SEM results. Due to the vast capability of RBCs to be adaptable, their state of well-being is a major indication for the general health status of an individual. We conclude that these changes, using an old technique in a novel application, may provide new insights and new avenues for future improvements in clinical medicine pertaining to conditions like COPD. |
| Databáze: | OpenAIRE |
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