Esophageal and Gastric Smooth Muscle Activity After Carbon Dioxide Pneumoperitoneum

Autor: Tutku Soyer, Hülya Akman, Zuhal Aktuna, Tolga Reşat Aydos, Murat Çakmak, Oğuzhan Korkut, Gökhan Osmanoğlu
Přispěvatelé: Kırıkkale Üniversitesi
Rok vydání: 2010
Předmět:
Zdroj: Journal of Surgical Research. 161:278-281
ISSN: 0022-4804
DOI: 10.1016/j.jss.2009.02.009
Popis: 9th Annual Meeting of the British-Association-of-Paediatric-Endoscopic-Surgeons -- OCT 08-10, 2007 -- Istanbul, TURKEY Aydos, Tolga Resat/0000-0002-1832-9336; Soyer, Tutku/0000-0003-1505-6042 WOS: 000277885300019 PubMed: 19524263 Background. The metabolic changes associated with carbon dioxide (CO(2)) pneumoperitoneum include metabolic acidosis and lowered intra-abdominal pH values. An experimental study was performed to evaluate the effect of CO(2) pneumoperitoneum on esophageal and gastric smooth muscle sensitivity in response to several agonists. Methods. Wistar albino rats, weighing 200-250 g, were allocated into three groups. After anesthetization with ketamine hydrochloride and xylazine, abdominal esophagus, gastroesophageal junction, and gastric fundus were removed via median laparotomy in the control group. In the oxygen (O(2)) group, a 16G catheter was inserted into the abdomen above the umbilicus and insufflated with 95% O(2) and 5% CO(2) with a pressure of 10 mm Hg. In the CO(2) group, CO(2) was insufflated at the same pressure within the same time and the tissues were removed at the end of a 60 min period of pneumoperitoneum. Abdominal esophageal segment (n:6), gastroesophageal junction (n:6) and gastric fundus (n:12) were suspended under 0.5 to 2 g resting tension in Tyrode solution in organ baths. Contraction responses were obtained by carbachol and serotonin and relaxation responses were evaluated by isoproterenol in each group. All the responses were compared by nonparametric Kruskal Wallis test. Results. Carbachol and serotonin induced contractile responses of abdominal segments, gastroesophageal junction, and gastric fundus showed no difference between the control, O(2), and CO(2) groups (P > 0.05). Isoproterenol relaxation responses of the three groups were also not statistically different from each other (P > 0.05). Conclusion. CO(2) pneumoperitoneum of 60 min has no influence on esophageal and gastric smooth muscle responses to different agonists in rats. (C) 2010 Elsevier Inc. All rights reserved. British Assoc Paediat Endoscop Surg
Databáze: OpenAIRE
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