Patient-derived xenograft in zebrafish embryos: a new platform for translational research in gastric cancer

Autor: Ping Wei, Chong-Yong Li, Jia-Qi Wu, Lizong Shen, Jing Zhai, Ai-Min Tan, Ming-Fang He
Rok vydání: 2017
Předmět:
Male
0301 basic medicine
Cancer Research
Pathology
Pyridines
Docetaxel
Translational Research
Biomedical
chemistry.chemical_compound
0302 clinical medicine
Apatinib
Zebrafish
biology
Middle Aged
lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens
Microinjection
Treatment Outcome
Oncology
030220 oncology & carcinogenesis
Female
Taxoids
Fluorouracil
medicine.drug
China
medicine.medical_specialty
animal structures
Microinjections
Cell Survival
Antineoplastic Agents
lcsh:RC254-282
03 medical and health sciences
Stomach Neoplasms
Patient-derived xenograft
In vivo
Cell Line
Tumor
medicine
Animals
Humans
Neoplasm Invasiveness
Aged
Cell Proliferation
Cell growth
Research
Cancer
Translational research
biology.organism_classification
medicine.disease
In vitro
Disease Models
Animal
030104 developmental biology
chemistry
Apoptosis
Cancer research
Gastric cancer
Neoplasm Transplantation
Zdroj: Journal of Experimental & Clinical Cancer Research : CR
Journal of Experimental & Clinical Cancer Research, Vol 36, Iss 1, Pp 1-10 (2017)
ISSN: 1756-9966
DOI: 10.1186/s13046-017-0631-0
Popis: Background Gastric cancer (GC) is among the most commonly cancer occurred in Asian, especially in China. With its high heterogeneity and few of validated drug targets, GC remains to be one of the most under explored areas of precision medicine. In this study, we aimed to establish an in vivo patient-derived xenograft (PDX) model based on zebrafish (Danio rerio) embryos, allowing for a rapid analysis of the angiogenic and invasive potentials, as well as a fast drug sensitivity testing. Methods Two human gastric cancer cell lines (AGS and SGC-7901) were xenografted into zebrafish embryos, their sensitivity to 5-FU were tested both in vitro and in vivo. Fourteen human primary cells from gastric cancer tissue were xenografted into zebrafish embryos, their proliferating, angiogenic and metastatic activities were evaluated in vivo. Sensitivity to 5-FU, docetaxel, and apatinib were also tested on primary samples from four patients. Results SGC-7901 showed higher sensitivity to 5-FU than AGS both in vitro (6.3 ± 0.9 μM vs.10.5 ± 1.8 μM) and in vivo. Nine out of fourteen patient samples were successfully transplanted in zebrafish embryos and all showed proliferating, angiogenic and metastatic potentials in the living embryos. Four cases showed varied sensitivity to the selected three chemotherapeutic drugs. Conclusions Our zebrafish PDX (zPDX) model is a preclinically reliable in vivo model for GC. The zPDX model is also a promising platform for the translational research and personalized treatment on GC. Electronic supplementary material The online version of this article (10.1186/s13046-017-0631-0) contains supplementary material, which is available to authorized users.
Databáze: OpenAIRE
Externí odkaz: